PMID- 10614772
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 66
IP  - 6
DP  - 1999 Dec
TI  - Trp-Lys-Tyr-Met-Val-D-Met is a chemoattractant for human phagocytic cells.
PG  - 915-22
AB  - Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm) is a synthetic peptide that stimulates 
      phosphoinositide (PI) hydrolysis in human leukocytes. The peptide binds to a 
      unique cell surface receptor(s). Recently we had demonstrated that human 
      neutrophils, monocytes, and B lymphocytes express this peptide-specific receptor 
      and that stimulation of human leukocytes with the peptide leads to activation of 
      the oxidative respiratory system and the bactericidal activity of neutrophils or 
      monocytes. In this study we showed that the peptide induces chemotaxis of 
      phagocytic leukocytes and studied the signaling pathway leading to chemotaxis in 
      human monocytes. The peptide-induced monocyte chemotaxis is pertussis toxin 
      (PTX)-sensitive. This fact correlates with the peptide's stimulation of PI 
      hydrolysis and intracellular Ca2+ ([Ca2+]i) release, which is also PTX-sensitive. 
      We demonstrate that the peptide-specific receptor is different from receptor(s) 
      for monocyte chemoattractant protein-1 (MCP-1). We also show that intracellular 
      signaling of WKYMVm leading to monocyte chemotaxis is different from that of 
      MCP-1. The peptide-mediated monocyte chemotaxis is insensitive to protein kinase 
      C (PKC) inhibitor (GF109203X) and butan-1-ol, ruling out PKC and phospholipase D 
      participation in this process. On the other hand, a tyrosine kinase inhibitor 
      (genistein) and RhoA inhibitor (C3 transferase) curtailed the peptide-induced 
      chemotaxis in a concentration-dependent manner, implying the involvement of 
      tyrosine kinase and RhoA, respectively. Treatment of human monocytes with the 
      peptide stimulates tyrosine phosphorylation of several cellular proteins, 
      including p125FAK and Pyk2 and translocation of RhoA from the cytosol to the 
      membrane. We conclude that WKYMVm induces chemotaxis of human phagocytic 
      leukocytes via unique receptors and signaling.
FAU - Bae, Y S
AU  - Bae YS
AD  - Department of Life Science, Pohang University of Science and Technology, Korea.
FAU - Kim, Y
AU  - Kim Y
FAU - Kim, Y
AU  - Kim Y
FAU - Kim, J H
AU  - Kim JH
FAU - Suh, P G
AU  - Suh PG
FAU - Ryu, S H
AU  - Ryu SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indoles)
RN  - 0 (Maleimides)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 0 (Oligopeptides)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Trp-Lys-Tyr-Met-Val-Met)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 8PJ61P6TS3 (1-Butanol)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 3.1.4.4 (Phospholipase D)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
RN  - L79H6N0V6C (bisindolylmaleimide I)
SB  - IM
MH  - 1-Butanol/pharmacology
MH  - B-Lymphocytes/cytology/drug effects/physiology
MH  - Chemokine CCL2/physiology
MH  - Chemotaxis, Leukocyte/*drug effects/physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - GTP-Binding Proteins/metabolism/physiology
MH  - Humans
MH  - Indoles/pharmacology
MH  - Maleimides/pharmacology
MH  - Monocyte Chemoattractant Proteins/*pharmacology
MH  - Monocytes/cytology/*drug effects/enzymology/physiology
MH  - Neutrophils/cytology/*drug effects/physiology
MH  - Oligopeptides/*pharmacology
MH  - Pertussis Toxin
MH  - Phospholipase D/antagonists & inhibitors/metabolism
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors/metabolism
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/physiology
MH  - Virulence Factors, Bordetella/pharmacology
MH  - rhoA GTP-Binding Protein/physiology
EDAT- 1999/12/30 00:00
MHDA- 1999/12/30 00:01
CRDT- 1999/12/30 00:00
PHST- 1999/12/30 00:00 [pubmed]
PHST- 1999/12/30 00:01 [medline]
PHST- 1999/12/30 00:00 [entrez]
AID - 10.1002/jlb.66.6.915 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1999 Dec;66(6):915-22. doi: 10.1002/jlb.66.6.915.