PMID- 10616535
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 116
IP  - 1
DP  - 2000 Jan 1
TI  - The application of comparative genomic hybridization to previously karyotyped 
      cervical cancer cell lines.
PG  - 59-65
AB  - This investigation is concerned with the application of comparative genomic 
      hybridization (CGH) to DNA from previously fully karyotyped cervical cancer cell 
      lines using G-banding and fluorescence in situ hybridization (FISH) to compare 
      the chromosome copy numbers observed in karyotypes with the profile shifts seen 
      in CGH analysis. It has demonstrated that diploid DNA can be used as a reference 
      to cohybridize with a test sample of any modal number because of the proportional 
      representation of every chromosome arm and region in equal volumes of both test 
      and reference DNAs. Profile shifts in the near-diploid line gave a clear 
      indication of over and under-representation of either the whole or parts of 
      chromosome arms. In near-tetraploid samples, profile shifts, either gain or loss 
      due to copy number changes from four to five, five to six, or four to three were 
      smaller and were not always seen; however, the points of profile shift would have 
      allowed us to work out most of the breakpoints if karyotype information had not 
      been available. The profiles, however, did not provide accurate information on 
      the ploidy status; this would need to be measured by other means for the CGH data 
      to be interpreted correctly. The 3q and 8q gain in all the squamous cell 
      carcinoma cell lines appeared very clearly. Comparative genomic hybridization 
      revealed a new breakpoint at 7q31 which was not detected originally on the 
      karyotype in DE3. A breakpoint on 9q was reassigned on the basis of the profile 
      shift from 9q13 to 9q22 in JE6. Clarification of the origin of a small fragment 
      from chromosome 20 constantly present in JE6 showed it to be 20q22-qter.
FAU - Thein, A
AU  - Thein A
AD  - Academic Department of Obstetrics & Gynaecology, St. Michael's Hospital, 
      University of Bristol, United Kingdom.
FAU - Trkova, M
AU  - Trkova M
FAU - Fox, M
AU  - Fox M
FAU - Parrington, J
AU  - Parrington J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Chromosome Banding
MH  - Chromosome Breakage
MH  - Chromosomes, Human, Pair 20
MH  - Chromosomes, Human, Pair 7
MH  - Chromosomes, Human, Pair 9
MH  - DNA
MH  - Diploidy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Karyotyping
MH  - *Nucleic Acid Hybridization
MH  - Translocation, Genetic
MH  - Tumor Cells, Cultured
MH  - Uterine Cervical Neoplasms/*genetics
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - S0165-4608(99)00108-9 [pii]
AID - 10.1016/s0165-4608(99)00108-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Jan 1;116(1):59-65. doi: 
      10.1016/s0165-4608(99)00108-9.