PMID- 10617214
OWN - NLM
STAT- MEDLINE
DCOM- 20000330
LR  - 20190721
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 41
IP  - 6
DP  - 1999 Dec
TI  - Sensitizing potential in mice, guinea pig and man of the preservative Euxyl K 400 
      and its ingredient methyldibromo glutaronitrile.
PG  - 330-8
AB  - The allergenicity of the preservative Euxyl K 400 and its principal allergen 
      methyldibromo glutaronitrile (MDBGN) (1,2-dibromo-2,4-dicyanobutane) was 
      investigated using 3 animal models; in mice, the local lymph node assay (LLNA) 
      and in guinea pigs, the guinea pig maximization test (GPMT) and the cumulative 
      contact enhancement test (CCET) with a dose-response protocol included. Previous 
      attempts to define the sensitization capacity of these chemicals have given 
      conflicting results. For comparison, the frequency and causes of positive patch 
      test reactions to Euxyl K 400 and MDBGN were studied in patients referred to an 
      occupational dermatology clinic. This investigation showed that Euxyl K 400 and 
      MDBGN can give rise to contact allergy in man and that the relevant cases found 
      mainly had similar exposure as non-occupational cases. A contact allergenic 
      potential could be detected for MDBGN in 2 animal models, i.e., the CCET and the 
      LLNA, and also for Euxyl K 400 in the LLNA. However, statistical analysis of the 
      results from the GPMT with MDBGN failed to detect the sensitizing potential of 
      this particular allergen. The results indicate that to be able to detect the 
      allergenic potential of Euxyl K 400 and MDBGN, a predictive test method with 
      multiple topical applications at induction is required. It is therefore important 
      that an investigator is aware of the possibility of using various predictive test 
      models for investigation of potential contact allergens.
FAU - Wahlkvist, H
AU  - Wahlkvist H
AD  - Dermatology Division, National Institute for Working Life, Stockholm, Sweden.
FAU - Boman, A
AU  - Boman A
FAU - Montelius, J
AU  - Montelius J
FAU - Wahlberg, J E
AU  - Wahlberg JE
LA  - eng
PT  - Journal Article
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Allergens)
RN  - 0 (Nitriles)
RN  - 0 (Preservatives, Pharmaceutical)
RN  - YX089CPS05 (1,2-dibromo-2,4-dicyanobutane)
SB  - IM
MH  - Allergens/*adverse effects
MH  - Animals
MH  - Dermatitis, Allergic Contact/*etiology
MH  - Dermatitis, Occupational/*etiology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Guinea Pigs
MH  - Humans
MH  - Lymph Nodes/pathology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Nitriles/*adverse effects
MH  - Patch Tests
MH  - Preservatives, Pharmaceutical/*adverse effects
MH  - Random Allocation
EDAT- 2000/01/05 09:00
MHDA- 2000/04/01 09:00
CRDT- 2000/01/05 09:00
PHST- 2000/01/05 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/01/05 09:00 [entrez]
AID - 10.1111/j.1600-0536.1999.tb06182.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 1999 Dec;41(6):330-8. doi: 
      10.1111/j.1600-0536.1999.tb06182.x.