PMID- 10617997
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20180510
IS  - 0002-9165 (Print)
IS  - 0002-9165 (Linking)
VI  - 71
IP  - 1 Suppl
DP  - 2000 Jan
TI  - n-3 polyunsaturated fatty acids inhibit the antigen-presenting function of human 
      monocytes.
PG  - 357S-60S
LID - 10.1093/ajcn/71.1.357s [doi]
AB  - Diets rich in n-3 polyunsaturated fatty acids (PUFAs) are associated with 
      suppression of cell-mediated immune responses, but the mechanisms are unclear. We 
      hypothesized that n-3 PUFAs can inhibit the function of human antigen-presenting 
      cells. A prerequisite for this role of blood monocytes is the cell surface 
      expression of major histocompatibility complex (MHC) class II molecules [human 
      leukocyte antigen (HLA)-DR, -DP, and -DQ], aided by the presence of intercellular 
      adhesion molecule-1 (ICAM-1) and leukocyte function associated antigens 1 and 3. 
      We showed previously that the n-3 PUFA eicosapentaenoic acid (EPA) inhibits the 
      expression of HLA-DR on unstimulated human monocytes in vitro, but that 
      docosahexaenoic acid (DHA) enhances its expression. However, both n-3 PUFAs 
      suppress the expression of HLA-DR, HLA-DP, and ICAM-1 on 
      interferon-gamma-activated monocytes. We also established that dietary fish-oil 
      supplementation can inhibit the expression of these surface molecules on 
      circulating human monocytes. We subsequently showed that when EPA and DHA were 
      combined in the same ratio as is commonly found in fish-oil-supplement capsules 
      (3:2), there was no significant effect in vitro on the expression of HLA-DR on 
      unstimulated monocytes, but the expression on activated monocytes remained 
      significantly inhibited. In the same in vitro system, the ability of activated 
      monocytes to present antigen to autologous lymphocytes was significantly reduced 
      after culture with the combined n-3 PUFAs. These findings provide one potential 
      mechanism for the beneficial effect of fish oil in the treatment of rheumatoid 
      arthritis, a disorder associated with elevated expression of MHC class II and 
      adhesion molecules on monocytes present within affected joints.
FAU - Hughes, D A
AU  - Hughes DA
AD  - Diet, Health and Consumer Science Division, the Institute of Food Research, 
      Norwich Research Park, Colney, Norwich, Norfolk, NR4 7UA, United Kingdom. 
      david.hughes@bbsrc.ac.uk
FAU - Pinder, A C
AU  - Pinder AC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Clin Nutr
JT  - The American journal of clinical nutrition
JID - 0376027
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Dietary Fats, Unsaturated)
RN  - 0 (Fatty Acids, Omega-3)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal
MH  - Antigen-Presenting Cells/*immunology
MH  - Arthritis, Rheumatoid/diet therapy/immunology
MH  - Dietary Fats, Unsaturated/*immunology
MH  - Docosahexaenoic Acids/immunology
MH  - Eicosapentaenoic Acid/immunology
MH  - Fatty Acids, Omega-3/*immunology
MH  - Female
MH  - Flow Cytometry
MH  - Genes, MHC Class II/immunology
MH  - Humans
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*immunology
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
AID - 10.1093/ajcn/71.1.357s [doi]
PST - ppublish
SO  - Am J Clin Nutr. 2000 Jan;71(1 Suppl):357S-60S. doi: 10.1093/ajcn/71.1.357s.