PMID- 10618174
OWN - NLM
STAT- MEDLINE
DCOM- 20000217
LR  - 20170531
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 34
IP  - 4
DP  - 1999
TI  - Benzene increases aneuploidy in the lymphocytes of exposed workers: a comparison 
      of data obtained by fluorescence in situ hybridization in interphase and 
      metaphase cells.
PG  - 260-8
AB  - Benzene is an established human leukemogen that increases the level of chromosome 
      aberrations in lymphocytes of exposed workers. Numerical aberrations (aneusomy) 
      can be observed by fluorescence in situ hybridization (FISH) in both interphase 
      and metaphase cells. Whereas interphase FISH allows nondividing cells to be 
      analyzed, one advantage of metaphase FISH is that it can also detect structural 
      changes. The present study compares the abilities of metaphase and interphase 
      FISH to detect aneusomy of chromosomes 7 and 8 in healthy benzene-exposed human 
      subjects. Metaphase and interphase cells from the peripheral blood of 43 workers 
      exposed to benzene (median = 31 ppm, 8-hr TWA) and 44 frequency-matched controls 
      were analyzed by FISH. Normal diploid cells contained two hybridization signals, 
      whereas those that were potentially monosomic contained one, trisomic 3 and 
      tetrasomic 4. The frequency of cells with one hybridization signal for chromosome 
      7 in metaphase spreads rose from 2.72 +/- 0.19 (%, mean +/- SE) in controls to 
      3.79 +/- 0.63 in workers exposed to 31 or fewer ppm benzene and 5.9 +/- 0.85 in 
      those exposed to more than 31 ppm (P(trend) < 0.0001). No similar dose-dependent 
      increase in the frequency of cells with one hybridization signal was observed by 
      interphase FISH, probably because of probe overlap artifact. Although significant 
      dose-dependent increases in the frequency of cells with three hybridization 
      signals for chromosome 7 were detected by both methods in the higher-exposed 
      group, a larger, more significant difference was detected by metaphase FISH 
      between controls and workers exposed to 31 or fewer ppm. Similar data were 
      obtained for chromosome 8. Interphase and metaphase FISH were moderately 
      correlated for three hybridization signals but not for one hybridization signal 
      in chromosome 7 or 8. In general, metaphase FISH was more sensitive in detecting 
      both monosomy and trisomy in the lymphocytes of exposed workers.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Zhang, L
AU  - Zhang L
AD  - School of Public Health, University of California, Berkeley, California, USA.
FAU - Rothman, N
AU  - Rothman N
FAU - Wang, Y
AU  - Wang Y
FAU - Hayes, R B
AU  - Hayes RB
FAU - Yin, S
AU  - Yin S
FAU - Titenko-Holland, N
AU  - Titenko-Holland N
FAU - Dosemeci, M
AU  - Dosemeci M
FAU - Wang, Y Z
AU  - Wang YZ
FAU - Kolachana, P
AU  - Kolachana P
FAU - Lu, W
AU  - Lu W
FAU - Xi, L
AU  - Xi L
FAU - Li, G L
AU  - Li GL
FAU - Smith, M T
AU  - Smith MT
LA  - eng
GR  - P42 ES004705/ES/NIEHS NIH HHS/United States
GR  - P30 ES01896/ES/NIEHS NIH HHS/United States
GR  - P42 ES04705/ES/NIEHS NIH HHS/United States
GR  - R01 ES06721/ES/NIEHS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - J64922108F (Benzene)
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Benzene/*toxicity
MH  - Case-Control Studies
MH  - Chromosomes, Human, Pair 7
MH  - Chromosomes, Human, Pair 8
MH  - Environmental Monitoring/methods
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase/drug effects/genetics
MH  - Lymphocytes/drug effects/physiology
MH  - Male
MH  - Metaphase/drug effects/genetics
MH  - *Occupational Exposure
MH  - Sensitivity and Specificity
MH  - Trisomy
EDAT- 2000/01/05 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/05 09:00
PHST- 2000/01/05 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/05 09:00 [entrez]
AID - 10.1002/(SICI)1098-2280(1999)34:4<260::AID-EM6>3.0.CO;2-P [pii]
PST - ppublish
SO  - Environ Mol Mutagen. 1999;34(4):260-8.