PMID- 10619861
OWN - NLM
STAT- MEDLINE
DCOM- 20000128
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 105
IP  - 1
DP  - 2000 Jan
TI  - In vivo expression of a new hepatitis B virus protein encoded by a spliced RNA.
PG  - 55-60
AB  - Hepatitis B virus (HBV) is a small DNA virus with a compact genomic organization. 
      All HBV proteins identified to date have been encoded by unspliced HBV RNAs. 
      Spliced HBV RNAs have been described, but their functions are unknown. We show 
      here that a singly spliced HBV RNA encodes a novel HBV protein in vivo. This HBV 
      splice-generated protein (HBSP) corresponds to the fusion of a part of the viral 
      polymerase and a new open reading frame that is created by the splicing event. In 
      vivo, HBSP protein was found in HBV-infected liver samples, and anti-HBSP 
      antibodies occurred in one-third of sera samples collected from chronic HBV 
      carriers. In vitro, the ectopic expression of HBSP had no effect on viral DNA 
      replication or transcription but induced cell apoptosis without a cell-cycle 
      block. Overall, our results suggest that HBV has evolved a mechanism that 
      directly modulates virus-cell interaction through RNA splicing.
FAU - Soussan, P
AU  - Soussan P
AD  - Institut National de la Sante et de la Recherche Medicale U370, CHU Necker, 75015 
      Paris, France.
FAU - Garreau, F
AU  - Garreau F
FAU - Zylberberg, H
AU  - Zylberberg H
FAU - Ferray, C
AU  - Ferray C
FAU - Brechot, C
AU  - Brechot C
FAU - Kremsdorf, D
AU  - Kremsdorf D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Cells, Cultured
MH  - DNA Replication
MH  - Hepatitis B virus/*chemistry
MH  - Hepatitis B, Chronic/*metabolism
MH  - Humans
MH  - Liver/chemistry
MH  - *RNA Splicing
MH  - Viral Proteins/*analysis/genetics
MH  - Virus Replication
PMC - PMC382588
EDAT- 2000/01/05 00:00
MHDA- 2000/01/05 00:01
PMCR- 2000/01/01
CRDT- 2000/01/05 00:00
PHST- 2000/01/05 00:00 [pubmed]
PHST- 2000/01/05 00:01 [medline]
PHST- 2000/01/05 00:00 [entrez]
PHST- 2000/01/01 00:00 [pmc-release]
AID - 08098 [pii]
AID - 10.1172/JCI8098 [doi]
PST - ppublish
SO  - J Clin Invest. 2000 Jan;105(1):55-60. doi: 10.1172/JCI8098.