PMID- 10620011
OWN - NLM
STAT- MEDLINE
DCOM- 20000225
LR  - 20191103
IS  - 1356-9597 (Print)
IS  - 1356-9597 (Linking)
VI  - 4
IP  - 11
DP  - 1999 Nov
TI  - Long range interaction of cis-DNA elements mediated by architectural 
      transcription factor Bach1.
PG  - 643-55
AB  - BACKGROUND: A central question in vertebrate transcriptional regulation is how 
      cis-regulatory modules, including enhancers, silencers and promoters, communicate 
      with each other over long distances to mandate proper gene expression. In order 
      to address this question we analysed protein/DNA interactions in the human 
      beta-globin locus control region (LCR). One of the many proteins that are 
      potentially implicated in LCR function is Bach1. Bach1 possesses a basic leucine 
      zipper (bZip) domain, as well as a BTB/POZ domain that has been shown to be 
      involved in the regulation of chromatin structure. Bach1 forms heterodimers with 
      small Maf proteins through its leucine zipper and binds to Maf recognition 
      elements (MARE). RESULTS: Using atomic force microscopy we visualized large 
      looped DNA structures between MAREs located in different regulatory elements 
      within the human beta-globin LCR that were mediated by Bach1/MafK heterodimers. 
      The formation of these DNA loops required the Bach1 BTB/POZ protein interaction 
      domain. Furthermore, in transfection studies we found that Bach1 repressed the 
      enhancer activity of the LCR in a BTB/POZ domain-dependent manner. CONCLUSION: 
      Our results suggest that Bach1 and other BTB/POZ transcription factors may 
      represent a class of nuclear architectural proteins that mediate long range 
      interactions between cis-regulatory elements in order to regulate gene 
      expression.
FAU - Yoshida, C
AU  - Yoshida C
AD  - Department of Biochemistry, Tohoku University School of Medicine, Sendai 
      980-8575, Japan.
FAU - Tokumasu, F
AU  - Tokumasu F
FAU - Hohmura, K I
AU  - Hohmura KI
FAU - Bungert, J
AU  - Bungert J
FAU - Hayashi, N
AU  - Hayashi N
FAU - Nagasawa, T
AU  - Nagasawa T
FAU - Engel, J D
AU  - Engel JD
FAU - Yamamoto, M
AU  - Yamamoto M
FAU - Takeyasu, K
AU  - Takeyasu K
FAU - Igarashi, K
AU  - Igarashi K
LA  - eng
GR  - R01 DK052356/DK/NIDDK NIH HHS/United States
GR  - R01 DK052356-04/DK/NIDDK NIH HHS/United States
GR  - R01 HL024415/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Genes Cells
JT  - Genes to cells : devoted to molecular & cellular mechanisms
JID - 9607379
RN  - 0 (ABTB1 protein, human)
RN  - 0 (BACH1 protein, human)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (Fanconi Anemia Complementation Group Proteins)
RN  - 0 (Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Basic-Leucine Zipper Transcription Factors
MH  - DNA/*metabolism
MH  - *Enhancer Elements, Genetic
MH  - Fanconi Anemia Complementation Group Proteins
MH  - Humans
MH  - Leucine Zippers/genetics
MH  - Locus Control Region/genetics
MH  - Models, Genetic
MH  - Models, Molecular
MH  - *Nucleic Acid Conformation
MH  - Protein Biosynthesis
MH  - Protein Structure, Tertiary/genetics
MH  - Proteins/physiology
MH  - Repressor Proteins/genetics/physiology
MH  - Transcription Factors/*physiology
MH  - Zinc Fingers/genetics
EDAT- 2000/01/05 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/05 09:00
PHST- 2000/01/05 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/05 09:00 [entrez]
AID - gtc291 [pii]
AID - 10.1046/j.1365-2443.1999.00291.x [doi]
PST - ppublish
SO  - Genes Cells. 1999 Nov;4(11):643-55. doi: 10.1046/j.1365-2443.1999.00291.x.