PMID- 10623558
OWN - NLM
STAT- MEDLINE
DCOM- 20000207
LR  - 20031114
IS  - 0882-4010 (Print)
IS  - 0882-4010 (Linking)
VI  - 28
IP  - 1
DP  - 2000 Jan
TI  - Monoclonal anti-LPS inner core antibodies protect against experimental 
      hematogenous Haemophilus influenzae type b meningitis.
PG  - 1-8
AB  - This study tested the protective activity of antibodies to the LPS core of 
      Haemophilus influenzae (Borrelli et al., Infect. Immun. 1995;63: 3683-92) in a 
      hematogenous meningitis model. Meningitis was established by intraperitoneal 
      inoculation of infant rats with H. influenzae type b (Hib). The severity of 
      infection was determined by daily assessment of mortality, symptoms of disease 
      and weight changes. Mortality occurred rapidly after infection with 10(5)cfu/rat 
      and most animals died within 24 h. At a lower infection dose (10(4)cfu/rat) the 
      rats survived, but developed symptoms of disease such as tremor, hypothermia, 
      lethargy and anorexia within 12-72 h post challenge. Surviving animals showed 
      decreased weight gain. Bacteremia was detected by daily blood-cultures in 10/10 
      rats and cleared 6 days after inoculation. The monoclonal anti-LPS antibody MAHI 
      3 was used in passive protection studies. MAHI 3 increased the survival in the 
      high inoculum group (10(5)cfu/rat) from 10-17% in control animals to 60-90%. At 
      the lower inoculum concentration (10(4)cfu/rat) MAHI 3 treatment reduced the 
      symptoms and blood counts. Intraperitoneal injection of MAHI 3 was more effective 
      than intranasal injection as shown by the effect on bacteremia. We conclude that 
      anti-LPS antibodies can protect against mortality caused by hematogenous Hib 
      infections in infant rats.
CI  - Copyright 2000 Academic Press.
FAU - Borrelli, S
AU  - Borrelli S
AD  - Divisions of Clinical & Oral Bacteriology, Karolinska Institute, Huddinge, 86, 
      S-141, Sweden.
FAU - Diab, A
AU  - Diab A
FAU - Lindberg, A
AU  - Lindberg A
FAU - Svanborg, C
AU  - Svanborg C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*therapeutic use
MH  - Bacteremia/prevention & control
MH  - Haemophilus influenzae/*immunology
MH  - Lipopolysaccharides/*immunology
MH  - Meningitis, Haemophilus/*prevention & control
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Weight Gain
EDAT- 2000/01/07 00:00
MHDA- 2000/01/07 00:01
CRDT- 2000/01/07 00:00
PHST- 2000/01/07 00:00 [pubmed]
PHST- 2000/01/07 00:01 [medline]
PHST- 2000/01/07 00:00 [entrez]
AID - S0882-4010(99)90318-2 [pii]
AID - 10.1006/mpat.1999.0318 [doi]
PST - ppublish
SO  - Microb Pathog. 2000 Jan;28(1):1-8. doi: 10.1006/mpat.1999.0318.