PMID- 10623560
OWN - NLM
STAT- MEDLINE
DCOM- 20000207
LR  - 20061115
IS  - 0882-4010 (Print)
IS  - 0882-4010 (Linking)
VI  - 28
IP  - 1
DP  - 2000 Jan
TI  - Induction of monocyte chemoattractant protein-1 (MCP-1) production by Pseudomonas 
      nitrite reductase in human pulmonary type II epithelial-like cells.
PG  - 17-23
AB  - Monocyte chemoattractant protein-1 (MCP-1), a chemoattractant for monocytes, is 
      presumed to play a pivotal role in the recruitment and accumulation of monocytes 
      in various diseases including pulmonary infections. We examined here whether or 
      not Pseudomonas nitrite reductase (PNR), a recently identified IL-8 inducer in 
      various respiratory cells, could stimulate human pulmonary type II 
      epithelial-like cells (A549) to induce MCP-1 production. A time- and 
      dose-dependent induction of MCP-1 protein synthesis associated with an increase 
      of MCP-1 mRNA expression by A549 cells was observed in response to PNR. New 
      protein translation was not required for PNR-mediated MCP-1 mRNA expression in 
      the same cells. When anti-human MCP-1 monoclonal antibody was used for 
      neutralizing of monocyte chemotactic factor (MCF) activities in the culture 
      supernatants of these cells stimulated with PNR, significant reductions of MCF 
      activities (the mean reduction rate; 49-59%, P<0. 05) were observed. These data 
      suggest that PNR may contribute to monocyte migration, through inducing pulmonary 
      epithelial cell-derived MCP-1 production in the airway of patients with pneumonia 
      due to P. aeruginosa.
CI  - Copyright 2000 Academic Press.
FAU - Sar, B
AU  - Sar B
AD  - Department of Internal Medicine, Institute of Tropical Medicine, 1-12-4 Sakamoto, 
      Nagasaki, 852-8523, Japan.
FAU - Oishi, K
AU  - Oishi K
FAU - Wada, A
AU  - Wada A
FAU - Hirayama, T
AU  - Hirayama T
FAU - Matsushima, K
AU  - Matsushima K
FAU - Nagatake, T
AU  - Nagatake T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - EC 1.7.- (Nitrite Reductases)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Epithelial Cells/metabolism
MH  - Humans
MH  - Lung/*metabolism
MH  - Nitrite Reductases/*physiology
MH  - Pseudomonas aeruginosa/*enzymology
MH  - RNA, Messenger/analysis
EDAT- 2000/01/07 00:00
MHDA- 2000/01/07 00:01
CRDT- 2000/01/07 00:00
PHST- 2000/01/07 00:00 [pubmed]
PHST- 2000/01/07 00:01 [medline]
PHST- 2000/01/07 00:00 [entrez]
AID - S0882-4010(99)90320-0 [pii]
AID - 10.1006/mpat.1999.0320 [doi]
PST - ppublish
SO  - Microb Pathog. 2000 Jan;28(1):17-23. doi: 10.1006/mpat.1999.0320.