PMID- 10623609
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 267
IP  - 1
DP  - 2000 Jan 7
TI  - Cloning and expression study of the mouse tetrodotoxin-resistant voltage-gated 
      sodium channel alpha subunit NaT/Scn11a.
PG  - 271-7
AB  - We have cloned a tetrodotoxin-resistant (TTX-R) voltage-gated sodium channel 
      alpha subunit from a mouse cDNA library and designated it as NaT. It encodes 1765 
      amino acid residues and is virtually identical to that of Scn11a, which has been 
      reported recently, except for 40 nt and 14 aa substitutions. The amino acid 
      identity of NaT/Scn11a with rat NaN/SNS2 is 88%. NaT/Scn11a was mapped to mouse 
      chromosome 9F3-F4 by fluorescence in situ hybridization (FISH). While rat 
      NaN/SNS2 has been reported to be expressed specifically in the peripheral sensory 
      neurons, NaT/Scn11a is expressed not only in the peripheral sensory neurons but 
      also in the spinal cord, uterus, testis, ovary, placenta, and small intestine. 
      NaT is detectable in mouse embryos 15 days postcoitus (p.c.), around the phase of 
      organogenesis and gonadal differentiation. These findings demonstrate a unique 
      distribution of NaT/Scn11a and suggest some of its roles in the above-mentioned 
      processes.
CI  - Copyright 2000 Academic Press.
FAU - Ogata, K
AU  - Ogata K
AD  - Graduate School of Medicine, University of Tokyo, Tokyo, 113-8655, Japan.
FAU - Jeong, S Y
AU  - Jeong SY
FAU - Murakami, H
AU  - Murakami H
FAU - Hashida, H
AU  - Hashida H
FAU - Suzuki, T
AU  - Suzuki T
FAU - Masuda, N
AU  - Masuda N
FAU - Hirai, M
AU  - Hirai M
FAU - Isahara, K
AU  - Isahara K
FAU - Uchiyama, Y
AU  - Uchiyama Y
FAU - Goto, J
AU  - Goto J
FAU - Kanazawa, I
AU  - Kanazawa I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Recombinant Proteins)
RN  - 0 (Sodium Channels)
RN  - 4368-28-9 (Tetrodotoxin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - Embryonic and Fetal Development
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Gene Library
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neurons, Afferent/*metabolism
MH  - Organ Specificity
MH  - Peripheral Nerves/*metabolism
MH  - Pregnancy
MH  - Rats
MH  - Recombinant Proteins/chemistry/drug effects/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Sodium Channels/chemistry/*genetics/physiology
MH  - Tetrodotoxin/*pharmacology
EDAT- 2000/01/07 00:00
MHDA- 2000/01/07 00:01
CRDT- 2000/01/07 00:00
PHST- 2000/01/07 00:00 [pubmed]
PHST- 2000/01/07 00:01 [medline]
PHST- 2000/01/07 00:00 [entrez]
AID - S0006-291X(99)91858-2 [pii]
AID - 10.1006/bbrc.1999.1858 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Jan 7;267(1):271-7. doi: 10.1006/bbrc.1999.1858.