PMID- 10623669
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 156
IP  - 1
DP  - 2000 Jan
TI  - Detection of Epstein-Barr virus in Hodgkin-Reed-Sternberg cells : no evidence for 
      the persistence of integrated viral fragments inLatent membrane protein-1 
      (LMP-1)-negative classical Hodgkin's disease.
PG  - 209-16
AB  - Classical Hodgkin's disease (HD) is associated with Epstein-Barr virus (EBV) 
      infection. Although in developing countries EBV can be demonstrated in 
      Hodgkin-Reed-Sternberg (H-RS) cells in up to 95% of HD cases, in industrialized 
      countries only about 50% of HD cases are associated with EBV. An open question 
      remains whether EBV in the EBV-negative cases has escaped detection by standard 
      screening procedures due to deletions in the viral genome associated with 
      integration of viral fragments into the host cell genome. We, among others, 
      recently described this phenomenon in Burkitt's lymphoma cells. To investigate 
      whether H-RS cells in latent membrane protein-1 (LMP-1)-negative HD cases harbor 
      fragments of the EBV genome, we combined fluorescence in situ hybridization 
      (FISH) using a set of six overlapping DNA probes spanning the whole EBV genome 
      with immunophenotyping of fresh frozen lymphoma sections. Results in the eight 
      cases analyzed were as follows: in three LMP-1-positive cases, FISH analysis 
      yielded specific signals for each EBV DNA probe in H-RS cells, which had been 
      identified by morphology and CD30 staining. In contrast, none of the EBV DNA 
      probes hybridized to the H-RS cells in the five LMP-1-negative cases. Thus, there 
      is no evidence for the presence of fragments of the viral genome integrated into 
      the host cell genome in the LMP-1-negative cases. Furthermore, in the 
      LMP-1-positive cases analyzed, no large deletions in the viral genome were 
      detected. These results show that, in classical HD, LMP-1-negative cases do not 
      harbor EBV DNA within the H-RS cells. Whether, in these cases, a still unknown 
      virus contributes to the transformation and maintenance of the malignant 
      phenotype remains to be established.
FAU - Staratschek-Jox, A
AU  - Staratschek-Jox A
AD  - Department of Internal Medicine I, University of Cologne, Cologne, Germany.
FAU - Kotkowski, S
AU  - Kotkowski S
FAU - Belge, G
AU  - Belge G
FAU - Rudiger, T
AU  - Rudiger T
FAU - Bullerdiek, J
AU  - Bullerdiek J
FAU - Diehl, V
AU  - Diehl V
FAU - Wolf, J
AU  - Wolf J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (DNA, Viral)
RN  - 0 (EBV-associated membrane antigen, Epstein-Barr virus)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - DNA, Viral/analysis
MH  - Female
MH  - Genome, Viral
MH  - Herpesvirus 4, Human/genetics/*isolation & purification
MH  - Hodgkin Disease/metabolism/*pathology/*virology
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Reed-Sternberg Cells/*virology
MH  - Viral Matrix Proteins/metabolism
MH  - Virion/isolation & purification
PMC - PMC1868626
EDAT- 2000/01/07 00:00
MHDA- 2000/01/07 00:01
PMCR- 2000/07/01
CRDT- 2000/01/07 00:00
PHST- 2000/01/07 00:00 [pubmed]
PHST- 2000/01/07 00:01 [medline]
PHST- 2000/01/07 00:00 [entrez]
PHST- 2000/07/01 00:00 [pmc-release]
AID - S0002-9440(10)64721-9 [pii]
AID - 2024 [pii]
AID - 10.1016/S0002-9440(10)64721-9 [doi]
PST - ppublish
SO  - Am J Pathol. 2000 Jan;156(1):209-16. doi: 10.1016/S0002-9440(10)64721-9.