PMID- 10627565
OWN - NLM
STAT- MEDLINE
DCOM- 20000207
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 3
DP  - 2000 Feb
TI  - Cloning the antibody response in humans with chronic inflammatory disease: 
      immunopanning of subacute sclerosing panencephalitis (SSPE) brain sections with 
      antibody phage libraries prepared from SSPE brain enriches for antibody 
      recognizing measles virus antigens in situ.
PG  - 1533-7
AB  - In central nervous system (CNS) infectious and inflammatory diseases of known 
      cause, oligoclonal bands represent antibody directed against the causative agent. 
      To determine whether disease-relevant antibodies can be cloned from diseased 
      brain, we prepared an antibody phage display library from the brain of a human 
      with subacute sclerosing panencephalitis (SSPE), a chronic encephalitis caused by 
      measles virus, and selected the library against SSPE brain sections. Antibodies 
      that were retrieved reacted strongly with measles virus cell extracts by 
      enzyme-linked immunosorbent assay and were specific for the measles virus 
      nucleocapsid protein. These antibodies immunostained cells in different SSPE 
      brains but not in control brain. Our data provide the first demonstration that 
      diseased brain can be used to select in situ for antibodies directed against the 
      causative agent of disease and point to the potential usefulness of this approach 
      in identifying relevant antibodies in chronic CNS or systemic inflammatory 
      diseases of unknown cause.
FAU - Owens, G P
AU  - Owens GP
AD  - Departments of Neurology, University of Colorado Health Sciences Center, Denver, 
      Colorado 80262, USA.
FAU - Williamson, R A
AU  - Williamson RA
FAU - Burgoon, M P
AU  - Burgoon MP
FAU - Ghausi, O
AU  - Ghausi O
FAU - Burton, D R
AU  - Burton DR
FAU - Gilden, D H
AU  - Gilden DH
LA  - eng
GR  - P01 NS032623/NS/NINDS NIH HHS/United States
GR  - AI 39162/AI/NIAID NIH HHS/United States
GR  - NS 32623/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Viral)
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Peptide Library)
SB  - IM
MH  - Antibodies, Viral/*immunology/*isolation & purification
MH  - Antibody Specificity
MH  - Bacteriophages
MH  - Brain/*immunology/virology
MH  - Capsid/immunology
MH  - Cloning, Molecular
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoglobulin Fab Fragments/immunology
MH  - Measles virus/*immunology
MH  - Peptide Library
MH  - Subacute Sclerosing Panencephalitis/*immunology
PMC - PMC111489
EDAT- 2000/01/11 00:00
MHDA- 2000/01/11 00:01
PMCR- 2000/02/01
CRDT- 2000/01/11 00:00
PHST- 2000/01/11 00:00 [pubmed]
PHST- 2000/01/11 00:01 [medline]
PHST- 2000/01/11 00:00 [entrez]
PHST- 2000/02/01 00:00 [pmc-release]
AID - 1279 [pii]
AID - 10.1128/jvi.74.3.1533-1537.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Feb;74(3):1533-7. doi: 10.1128/jvi.74.3.1533-1537.2000.