PMID- 10630874
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20190720
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 864
IP  - 1
DP  - 1999 Dec 9
TI  - Determination of ochratoxin A in wine by means of immunoaffinity column clean-up 
      and high-performance liquid chromatography.
PG  - 89-101
AB  - A new and accurate method to quantify ochratoxin A (OA) in table wine has been 
      developed. The method uses commercial immunoaffinity columns for clean-up and 
      high-performance liquid chromatography (HPLC) with fluorescence detection for 
      quantification of the toxin. Wine was diluted with a solution containing 1% 
      polyethylene glycol (PEG 8000) and 5% sodium hydrogencarbonate, filtered and 
      applied to an OchraTest immunoaffinity column. The column was washed with a 
      solution containing sodium chloride (2.5%) and sodium hydrogencarbonate (0.5%) 
      followed by water. OA was eluted with methanol and quantified by reversed-phase 
      HPLC with fluorometric detection (excitation wavelength 333 nm, emission 
      wavelength 460 nm) using acetonitrile-water-acetic acid (99:99:2) as mobile 
      phase. Average recoveries of OA from white, rose and red wine samples spiked at 
      levels from 0.04 to 10 ng/ml ranged from 88% to 103%, with relative standard 
      deviations (RSDs) between 0.2 and 9.7%. Detection limit was 0.01 ng/ml based on a 
      signal-to-noise ratio of 3:1. The method was applied successfully to 56 samples 
      of red (38), rose (8), white (9) and dessert (1) wine. The levels of OA ranged 
      from <0.01 to 7.6 ng/ml with red wines more contaminated than rose and white 
      wines. A good correlation (r=0.987) was found by comparative analysis of 20 
      naturally contaminated samples using this method and the method of Zimmerli and 
      Dick with better recoveries of OA and better performances for the new method. 
      Several advantages of this method with respect to the actually available methods 
      have been pointed out, with particular reference to red wine which appears to be 
      the most difficult to analyze.
FAU - Visconti, A
AU  - Visconti A
AD  - Istituto Tossine e Micotossine do Parassiti Vegetali, CNR, Bari, Italy. 
      visconti@area.ba.cnr.it
FAU - Pascale, M
AU  - Pascale M
FAU - Centonze, G
AU  - Centonze G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Acetonitriles)
RN  - 0 (Carcinogens)
RN  - 0 (Mycotoxins)
RN  - 0 (Ochratoxins)
RN  - 059QF0KO0R (Water)
RN  - 1779SX6LUY (ochratoxin A)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - Q40Q9N063P (Acetic Acid)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetic Acid
MH  - Acetonitriles
MH  - Carcinogens/analysis
MH  - Chromatography, Affinity/*methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Hydrogen-Ion Concentration
MH  - Immunoassay
MH  - Mass Spectrometry
MH  - Mycotoxins/*analysis
MH  - Ochratoxins/*analysis
MH  - Polyethylene Glycols
MH  - Spectrometry, Fluorescence
MH  - Water
MH  - Wine/*analysis
EDAT- 2000/01/12 00:00
MHDA- 2000/01/12 00:01
CRDT- 2000/01/12 00:00
PHST- 2000/01/12 00:00 [pubmed]
PHST- 2000/01/12 00:01 [medline]
PHST- 2000/01/12 00:00 [entrez]
AID - S0021-9673(99)00996-6 [pii]
AID - 10.1016/s0021-9673(99)00996-6 [doi]
PST - ppublish
SO  - J Chromatogr A. 1999 Dec 9;864(1):89-101. doi: 10.1016/s0021-9673(99)00996-6.