PMID- 10630960
OWN - NLM
STAT- MEDLINE
DCOM- 20000218
LR  - 20190831
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 60
IP  - 3
DP  - 2000 Mar
TI  - Reactivation of human herpesvirus 6 by infection of human herpesvirus 7.
PG  - 284-9
AB  - We have attempted to reactivate human herpesvirus 6 (HHV-6) by infection with 
      HHV-7 using childhood exanthem subitum patients in vitro. Peripheral blood 
      mononuclear cells (PBMCs) were collected from children who had a history of 
      exanthem subitum(ES) by HHV-6 and were infected by human herpesvirus 7 (HHV-7) in 
      vitro. The antigen positive rate to HHV-6 started to increase 7 days after the 
      infection and reached a maximum by Day 15 using an immunofluorescence antibody 
      test. The copy number of HHV-6 DNA also increased in the samples in 10 days after 
      infection in vitro. No antigen or increase in DNA was detected in PBMCs, that 
      were mock-infected or infected with supernatant of stock virus after 
      ultracentrifugation, suggesting that an infection by HHV-7 is necessary to 
      reactivate HHV-6. In the paired sera samples during the acute and the 
      convalescent phases of ES, seven to ten bands, that were specific for HHV-6, were 
      recognized in samples from the acute phase, and at least 5 dominant polypeptides 
      were found more intensively after HHV-7 infection.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Tanaka-Taya, K
AU  - Tanaka-Taya K
AD  - Department of Developmental Medicine (Pediatrics) D-5, Osaka University Graduate 
      School of Medicine, Suita City, Osaka, Japan.
FAU - Kondo, T
AU  - Kondo T
FAU - Nakagawa, N
AU  - Nakagawa N
FAU - Inagi, R
AU  - Inagi R
FAU - Miyoshi, H
AU  - Miyoshi H
FAU - Sunagawa, T
AU  - Sunagawa T
FAU - Okada, S
AU  - Okada S
FAU - Yamanishi, K
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Blotting, Southern
MH  - Child, Preschool
MH  - DNA, Viral/analysis
MH  - Herpesviridae Infections/blood/*virology
MH  - Herpesvirus 6, Human/growth & development/*physiology
MH  - Herpesvirus 7, Human/*physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Infant
MH  - Leukocytes, Mononuclear/virology
MH  - Polymerase Chain Reaction
MH  - Precipitin Tests
MH  - *Virus Activation
EDAT- 2000/01/12 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/12 09:00
PHST- 2000/01/12 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/12 09:00 [entrez]
AID - 10.1002/(SICI)1096-9071(200003)60:3<284::AID-JMV6>3.0.CO;2-8 [pii]
AID - 10.1002/(sici)1096-9071(200003)60:3<284::aid-jmv6>3.0.co;2-8 [doi]
PST - ppublish
SO  - J Med Virol. 2000 Mar;60(3):284-9. doi: 
      10.1002/(sici)1096-9071(200003)60:3<284::aid-jmv6>3.0.co;2-8.