PMID- 10631114
OWN - NLM
STAT- MEDLINE
DCOM- 20000223
LR  - 20181130
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 267
IP  - 2
DP  - 2000 Jan 19
TI  - Interactions between cancer and bone marrow cells induce osteoclast 
      differentiation factor expression and osteoclast-like cell formation in vitro.
PG  - 632-7
AB  - Cancer cells metastasized to bone induce osteoclastogenesis for bone destruction. 
      Coculture of either mouse melanoma B16 or breast cancer Balb/c-MC cells with 
      mouse bone marrow cells (BMCs) induced osteoclast-like cells, which were not 
      observed when cancer cells were segregated from BMCs. Osteoclast differentiation 
      factor (ODF), also known as receptor activator of NF-kappaB ligand (RANKL), is a 
      direct mediator of many osteotropic factors. Neither BMCs, B16 nor Balb/c-MC 
      cells alone expressed ODF mRNA. However, coculture of these cancer cells with 
      BMCs induced ODF expression, which was prevented by indomethacin. Moreover, the 
      coculture with cancer cells inhibited secretion of 
      osteoprotegerin/osteoclastogenesis inhibitory factor (OPG/OCIF), an inhibitory 
      decoy receptor for ODF, from BMCs. Thus, enhanced osteoclastogenesis in the 
      presence of cancer cells might be due to an increase in ODF activity. These 
      results suggest that interactions between cancer cells and BMCs induce ODF 
      expression and suppress OPG/OCIF level in metastatic foci resulting in 
      pathological osteoclastogenesis for bone destruction.
CI  - Copyright 2000 Academic Press.
FAU - Chikatsu, N
AU  - Chikatsu N
AD  - Division of Endocrinology, University of Tokyo School of Medicine, Tokyo, 
      112-8688, Japan.
FAU - Takeuchi, Y
AU  - Takeuchi Y
FAU - Tamura, Y
AU  - Tamura Y
FAU - Fukumoto, S
AU  - Fukumoto S
FAU - Yano, K
AU  - Yano K
FAU - Tsuda, E
AU  - Tsuda E
FAU - Ogata, E
AU  - Ogata E
FAU - Fujita, T
AU  - Fujita T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Osteoprotegerin)
RN  - 0 (RANK Ligand)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Receptor Activator of Nuclear Factor-kappa B)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tnfrsf11a protein, mouse)
RN  - 0 (Tnfrsf11b protein, mouse)
RN  - 0 (Tnfsf11 protein, mouse)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Bone Marrow Cells/*pathology
MH  - Bone Neoplasms/pathology/*secondary
MH  - Carrier Proteins/*genetics
MH  - Cell Adhesion
MH  - Coculture Techniques
MH  - DNA Primers/genetics
MH  - Female
MH  - Gene Expression/drug effects
MH  - Glycoproteins/metabolism
MH  - Indomethacin/pharmacology
MH  - Male
MH  - Mammary Neoplasms, Experimental/genetics/pathology/secondary
MH  - Melanoma, Experimental/genetics/pathology/secondary
MH  - Membrane Glycoproteins/*genetics
MH  - Mice
MH  - Osteoclasts/drug effects/metabolism/*pathology
MH  - Osteoprotegerin
MH  - RANK Ligand
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Neoplasm/genetics/metabolism
MH  - Receptor Activator of Nuclear Factor-kappa B
MH  - *Receptors, Cytoplasmic and Nuclear
MH  - Receptors, Tumor Necrosis Factor
EDAT- 2000/01/13 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/13 09:00
PHST- 2000/01/13 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/13 09:00 [entrez]
AID - S0006-291X(99)92008-9 [pii]
AID - 10.1006/bbrc.1999.2008 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2000 Jan 19;267(2):632-7. doi: 
      10.1006/bbrc.1999.2008.