PMID- 10631669
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20181113
IS  - 1044-0305 (Print)
IS  - 1044-0305 (Linking)
VI  - 11
IP  - 1
DP  - 2000 Jan
TI  - Discerning matrix-cluster peaks in matrix-assisted laser desorption/ionization 
      time-of-flight mass spectra of dilute peptide mixtures.
PG  - 88-93
AB  - Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass 
      spectrometry is widely used for the analysis of peptide mixtures such as those 
      resulting from protein digestion. Among several useful peptide matrices, 
      alpha-cyano-4-hydroxycinnamic acid (4-HCCA) appears to be the most popular. This 
      matrix does not generally give matrix-cluster peaks at the mass region covered by 
      enzyme-digested peptides (i.e., m/z above approximately 500). However, when an 
      analyte mixture is very dilute and/or the sample contains a large amount of 
      salts, ion peaks from matrix clusters can be quite intense, compared to peptide 
      peaks. This matrix-cluster interference becomes more pronounced as the amount of 
      analyte decreases. In this paper, a simple scheme for matrix-cluster 
      identification is reported. It is shown that matrix-cluster formation follows a 
      systematic pattern, although the relative intensities of these cluster ions 
      cannot be predicted. Discerning the matrix-cluster peaks from the peptide peaks 
      is found to be critical in analyzing dilute peptide mixtures with both 
      conventional and microspot MALDI-TOF techniques.
FAU - Keller, B O
AU  - Keller BO
AD  - Department of Chemistry, University of Alberta, Edmonton, Canada.
FAU - Li, L
AU  - Li L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Am Soc Mass Spectrom
JT  - Journal of the American Society for Mass Spectrometry
JID - 9010412
RN  - 0 (Peptide Fragments)
RN  - 0 (Peptides)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 3.4.21.4 (Trypsin)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Hydrolysis
MH  - Peptide Fragments/chemistry
MH  - Peptides/*chemistry
MH  - Potassium/chemistry
MH  - Sodium/chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Trypsin
EDAT- 2000/01/13 00:00
MHDA- 2000/01/13 00:01
CRDT- 2000/01/13 00:00
PHST- 2000/01/13 00:00 [pubmed]
PHST- 2000/01/13 00:01 [medline]
PHST- 2000/01/13 00:00 [entrez]
AID - S1044-0305(99)00126-9 [pii]
AID - 10.1016/S1044-0305(99)00126-9 [doi]
PST - ppublish
SO  - J Am Soc Mass Spectrom. 2000 Jan;11(1):88-93. doi: 10.1016/S1044-0305(99)00126-9.