PMID- 10632481
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20231213
IS  - 0094-6176 (Print)
IS  - 0094-6176 (Linking)
VI  - 25
IP  - 6
DP  - 1999
TI  - Experiences of the Dresdner Cord Blood Bank, supported by the Deutsche 
      Knochenmarkspenderdatei.
PG  - 575-8
AB  - Allogeneic bone marrow and peripheral blood stem cell transplantation is the 
      treatment of choice for some malignant hematologic diseases, marrow failure 
      syndromes, and severe congenital immunodeficiency states. Since Gluckman et al 
      reported in 1988 the first successful human leukocyte antigen (HLA)-matched 
      sibling umbilical cord blood stem cell transplantation, it has been known that 
      cord blood is a valuable source of hematopoietic stem cells. The Cord Blood Bank 
      at the University Hospital of Dresden was founded in 1997 and started collecting, 
      processing, and cryoconserving umbilical cord blood in August 1997. The cord 
      blood bank is supported by the largest German donor registry: Deutsche 
      Knochenmarkspenderdatei (DKMS) in Tubingen, Germany. With the informed consent of 
      the mothers, the collection is performed in collaboration with six hospitals in 
      Dresden, Berlin, and Bautzen. We routinely perform a volume reduction by 
      centrifuging the blood bag and expressing the leukocyte-rich supernatant. 
      Routinely, sterility, total nucleated cells (TNC), CD34+ cell count, HLA class I 
      and II, ABO/Rh blood group, and colony-forming units are evaluated. The maternal 
      blood is screened for anti-immunodeficiency virus (anti-HIV), anti-hepatitis C 
      virus (anti-HCV), anti-hepatitis B surface antigen (HBsAg), anti-hepatitis B 
      surface (anti-HBs), anti-hepatitis B core (anti-HBc), anticytomegalovirus 
      (anti-CMV), and toxoplasmosis and with Treponema pallidum hemagglutination assay 
      (TPHA). More than 1,000 cord blood units could be collected. Because of the 
      required volume and cell count and because of sterility, 50% of the collected 
      units had to be discharged. Our results are comparable with data of other cord 
      blood banks: mean volume 79 mL; cell count after volume reduction-TNC, 7.16 x 
      10(8); mononucleated cells (MNC), 3.75 x 10(8); CD34+ cells, 1.95 x 10(6); 
      colony-forming units (CFU), 67.1 x 10(4). To increase the pool of potential 
      umbilical cord blood units and in order to evaluate the possibility for unrelated 
      transplants, cryopreservation and banking of large numbers of cord bloods are 
      necessary.
FAU - Ordemann, R
AU  - Ordemann R
AD  - Department of Medicine I, University Hospital Dresden, Germany.
FAU - Petzold, K
AU  - Petzold K
FAU - Holig, K
AU  - Holig K
FAU - Schaffer, B
AU  - Schaffer B
FAU - Mauersberger, S
AU  - Mauersberger S
FAU - Ehninger, G
AU  - Ehninger G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Semin Thromb Hemost
JT  - Seminars in thrombosis and hemostasis
JID - 0431155
SB  - IM
EIN - Semin Thromb Hemost 2000;26(2):followi. Ehminger G [corrected to Ehninger G]
MH  - Animals
MH  - Blood Banks/statistics & numerical data
MH  - Fetal Blood/cytology
MH  - Germany
MH  - Humans
MH  - Registries
MH  - Stem Cells
MH  - Blood Banking/*methods
EDAT- 2000/01/13 00:00
MHDA- 2000/01/13 00:01
CRDT- 2000/01/13 00:00
PHST- 2000/01/13 00:00 [pubmed]
PHST- 2000/01/13 00:01 [medline]
PHST- 2000/01/13 00:00 [entrez]
AID - 10.1055/s-2007-994968 [doi]
PST - ppublish
SO  - Semin Thromb Hemost. 1999;25(6):575-8. doi: 10.1055/s-2007-994968.