PMID- 10634171
OWN - NLM
STAT- MEDLINE
DCOM- 20000215
LR  - 20171116
IS  - 1525-8165 (Print)
IS  - 1525-8165 (Linking)
VI  - 8
IP  - 4
DP  - 1999 Aug
TI  - Hematopoietic reconstitution of syngeneic mice with a peripheral blood-derived, 
      monoclonal CD34-, Sca-1+, Thy-1(low), c-kit+ stem cell line.
PG  - 335-42
AB  - Previous work had revealed that a CD34- fibroblast-like cell is the earliest 
      hematopoietic progenitor population. This cell type is able to differentiate into 
      hematopoietic progeny of all lineages and circulates in the peripheral blood, 
      from where it can be isolated by IL-6-mediated plastic adherence. We isolated 
      peripheral blood-derived mononuclear cells (MNC) from male CBA mice and 
      established in vitro a fibroblast-like, adherent growing cell layer. Cells were 
      immortalized by SV-40 transfection for cellular cloning. Monoclonal 
      fibroblast-like cell clones were established, and the surface expression of early 
      stem cell markers was determined by flow cytometry. Clones were CD34-, Sca-1+, 
      Thy-1(low), and c-kit+. Lethally irradiated female CBA mice were successfully 
      transplanted with a fibroblast-like cell clone, R-M26/2-1. After syngeneic 
      transplantation, peripheral blood counts were back to normal in transplanted mice 
      on days 15-20, and fluorescence in situ hybridization (FISH) revealed the sole 
      presence of male hematopoietic cells in the BM of female recipients at weeks 7, 
      9, 11, and 16 after transplantation. Immunohistochemistry for the expression of 
      CD34, Sca-1, Thy-1, and c-kit showed the presence of the phenotype of the 
      transplanted stem cell clone along the bone spicules in the marrow cavity, giving 
      rise to HPC of all lineages. In summary, we have shown that a CD34-, Sca-1+, 
      Thy-1(low), and c-kit+ fibroblast-like cell is consistent with the phenotype of 
      the earliest hematopoietic and repopulating stem cell and can be isolated from 
      peripheral blood cells.
FAU - Lange, C
AU  - Lange C
AD  - Institute of Clinical Hematology, GSF, Munich, Germany.
FAU - Kaltz, C
AU  - Kaltz C
FAU - Thalmeier, K
AU  - Thalmeier K
FAU - Kolb, H J
AU  - Kolb HJ
FAU - Huss, R
AU  - Huss R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Hematother Stem Cell Res
JT  - Journal of hematotherapy & stem cell research
JID - 100892915
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, Ly)
RN  - 0 (Ly6a protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (Thy-1 Antigens)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
CIN - J Hematother Stem Cell Res. 2000 Oct;9(5):607-9. PMID: 11091483
MH  - Animals
MH  - Antigens, CD34
MH  - Antigens, Ly
MH  - Cell Differentiation
MH  - *Cell Lineage
MH  - Female
MH  - Fibroblasts/*pathology/transplantation
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/*pathology
MH  - Male
MH  - Membrane Proteins
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Proto-Oncogene Proteins c-kit
MH  - Thy-1 Antigens
MH  - Transplantation, Isogeneic
EDAT- 2000/01/14 09:00
MHDA- 2000/02/19 09:00
CRDT- 2000/01/14 09:00
PHST- 2000/01/14 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/01/14 09:00 [entrez]
AID - 10.1089/152581699320090 [doi]
PST - ppublish
SO  - J Hematother Stem Cell Res. 1999 Aug;8(4):335-42. doi: 10.1089/152581699320090.