PMID- 10637493
OWN - NLM
STAT- MEDLINE
DCOM- 20000202
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 14
IP  - 1
DP  - 2000 Jan
TI  - Ph-negative non-Hodgkin's lymphoma occurring in chronic phase of Ph-positive 
      chronic myelogenous leukemia is defined as a genetically different neoplasm from 
      extramedullary localized blast crisis: report of two cases and review of the 
      literature.
PG  - 169-82
AB  - This report describes two cases of Philadelphia chromosome-negative (Ph(-)) 
      non-Hodgkin's lymphomas (NHLs) recognized in patients with chronic phase 
      Ph-positive (Ph(+)) chronic myelogenous leukemia (CML). Lymph node biopsy of 
      patient 1 was initially diagnosed as diffuse large B cell non-Hodgkin's lymphoma 
      (NHL, T cell rich variant), but at relapse showed immunoblastic features with a 
      marked decrease of admixed lymphocyte components. Patient 2 presented with 
      thickened parietal pleura which revealed a CD30-positive anaplastic large cell 
      lymphoma showing null cell phenotype and genotype with abundant admixed 
      neutrophils and lymphocytes. At the time of lymphoma diagnosis, the patients had 
      CML for 33 and 10 months, respectively. DNA obtained from bone marrow cells at 
      the time of lymphoma diagnosis showed BCR/ABL gene rearrangements by both 
      Southern blot analysis and reverse transcription polymerase chain reaction 
      (RT-PCR), but lacked both immunoglobulin and T cell receptor gene rearrangements. 
      BCR gene rearrangement and BCR/ABL fusion gene were also identified in lymph node 
      and pleural biopsies by Southern blot and RT-PCR analysis, respectively. However, 
      both biopsy specimens also contained reactive lymphocytes and neutrophils, and no 
      fusion signals between BCR and ABL genes were identified in the hyperdiploid 
      lymphoma cells of either case by fluorescence in situ hybridization (FISH). These 
      data suggest the lymphoma cells in both cases were not genetically associated 
      with BCR/ABL. Therefore, these cases were not diagnosed as an extramedullary 
      localized blast crisis in CML, but as Ph(-) NHLs. This represents the first 
      definitive demonstration of peripheral B cell lymphoma occurring by a separate 
      genetic pathway, lacking BCR/ABL, in patients with Ph(+) CML. A review of the 
      literature identified two different subtypes of malignant lymphomas arising in 
      patients with an antecedent or concurrent diagnosis of CML. The most common are T 
      cell lymphomas displaying an immature thymic phenotype, while peripheral B cell 
      lymphomas are more rare. Our study shows, however, that 'Ph(+) NHL' occurring in 
      CML or acute lymphocytic leukemia (ALL) may represent an unrelated neoplasm, even 
      if standard cytogenetic analysis reveals a Ph(+) chromosome, and that FISH is 
      required to confirm whether a localized lymphoid neoplasm is either a true 
      extramedullary localized blast crisis or genetically distinct neoplasm. 
      Leukemia(2000) 14, 169-182.
FAU - Ichinohasama, R
AU  - Ichinohasama R
AD  - Department of Oral Pathology, Tohoku University School of Dentistry, Sendai, 
      Japan.
FAU - Miura, I
AU  - Miura I
FAU - Takahashi, N
AU  - Takahashi N
FAU - Sugawara, T
AU  - Sugawara T
FAU - Tamate, E
AU  - Tamate E
FAU - Endoh, K
AU  - Endoh K
FAU - Endoh, F
AU  - Endoh F
FAU - Naganuma, H
AU  - Naganuma H
FAU - DeCoteau, J F
AU  - DeCoteau JF
FAU - Griffin, J D
AU  - Griffin JD
FAU - Kadin, M E
AU  - Kadin ME
FAU - Ooya, K
AU  - Ooya K
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DNA Primers)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Base Sequence
MH  - DNA Primers
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Leukemia, Myeloid, Chronic-Phase/*genetics
MH  - Lymphoma, Non-Hodgkin/*genetics/immunology/pathology
MH  - Male
MH  - Middle Aged
MH  - *Philadelphia Chromosome
MH  - Reverse Transcriptase Polymerase Chain Reaction
RF  - 56
EDAT- 2000/01/19 00:00
MHDA- 2000/01/19 00:01
CRDT- 2000/01/19 00:00
PHST- 2000/01/19 00:00 [pubmed]
PHST- 2000/01/19 00:01 [medline]
PHST- 2000/01/19 00:00 [entrez]
AID - 10.1038/sj.leu.2401606 [doi]
PST - ppublish
SO  - Leukemia. 2000 Jan;14(1):169-82. doi: 10.1038/sj.leu.2401606.