PMID- 10640140
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 116
IP  - 2
DP  - 2000 Jan 15
TI  - A multicenter investigation with D-FISH BCR/ABL1 probes.
PG  - 97-104
AB  - Twenty-eight laboratories evaluated a new fluorescence in situ hybridization 
      (FISH) strategy for chronic myeloid leukemia. In a three-part study, bcr/abl1 
      D-FISH probes were used to study bone marrow specimens. First, laboratories 
      familiarized themselves with the strategy by applying it to known normal and 
      abnormal specimens. Then, collectively the laboratories studied 20 normal and 20 
      abnormal specimens blindly and measured workload. Finally, each laboratory and 
      two experts studied six serial dilutions with 98-0% abnormal nuclei. Using the 
      reported normal cutoff of < 1% abnormal nuclei, participants reported no 
      false-negative cases and 15 false-positive cases (1-6.6% abnormal nuclei). 
      Results provided by participants for serial dilutions approximated the expected 
      percentages of abnormal nuclei, but those from the experts exhibited greater 
      precision. The clinical sensitivity, precision, nomenclature, workload, 
      recommendations for training, and quality assurance in methods using D-FISH in 
      clinical practice are discussed.
FAU - Dewald, G
AU  - Dewald G
AD  - Cytogenetics Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
FAU - Stallard, R
AU  - Stallard R
FAU - Alsaadi, A
AU  - Alsaadi A
FAU - Arnold, S
AU  - Arnold S
FAU - Blough, R
AU  - Blough R
FAU - Ceperich, T M
AU  - Ceperich TM
FAU - Rafael Elejalde, B
AU  - Rafael Elejalde B
FAU - Fink, J
AU  - Fink J
FAU - Higgins, J V
AU  - Higgins JV
FAU - Higgins, R R
AU  - Higgins RR
FAU - Hoeltge, G A
AU  - Hoeltge GA
FAU - Hsu, W T
AU  - Hsu WT
FAU - Johnson, E B
AU  - Johnson EB
FAU - Kronberger, D
AU  - Kronberger D
FAU - McCorquodale, D J
AU  - McCorquodale DJ
FAU - Meisner, L F
AU  - Meisner LF
FAU - Micale, M A
AU  - Micale MA
FAU - Oseth, L
AU  - Oseth L
FAU - Payne, J S
AU  - Payne JS
FAU - Schwartz, S
AU  - Schwartz S
FAU - Sheldon, S
AU  - Sheldon S
FAU - Sophian, A
AU  - Sophian A
FAU - Storto, P
AU  - Storto P
FAU - Van Tuinen, P
AU  - Van Tuinen P
FAU - Wenger, G D
AU  - Wenger GD
FAU - Wiktor, A
AU  - Wiktor A
FAU - Willis, L A
AU  - Willis LA
FAU - Yung, J F
AU  - Yung JF
FAU - Zenger-Hain, J
AU  - Zenger-Hain J
LA  - eng
GR  - MCJ-551004-05/PHS HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Fluorescent Dyes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Bone Marrow/pathology
MH  - Clinical Laboratory Techniques/*standards
MH  - Fluorescent Dyes
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/instrumentation/methods/standards
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/genetics/pathology
MH  - Quality Control
MH  - Sensitivity and Specificity
MH  - Workload
EDAT- 2000/01/20 00:00
MHDA- 2000/01/20 00:01
CRDT- 2000/01/20 00:00
PHST- 2000/01/20 00:00 [pubmed]
PHST- 2000/01/20 00:01 [medline]
PHST- 2000/01/20 00:00 [entrez]
AID - S016546089900120X [pii]
AID - 10.1016/s0165-4608(99)00120-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Jan 15;116(2):97-104. doi: 
      10.1016/s0165-4608(99)00120-x.