PMID- 10640185
OWN - NLM
STAT- MEDLINE
DCOM- 20000208
LR  - 20190910
IS  - 0300-4864 (Print)
IS  - 0300-4864 (Linking)
VI  - 27
IP  - 4
DP  - 1998 Apr
TI  - Differential expression of the L- and S-isoforms of myelin associated 
      glycoprotein (MAG) in oligodendrocyte unit phenotypes in the adult rat anterior 
      medullary velum.
PG  - 271-80
AB  - We have previously demonstrated differences in the expression of carbonic 
      anhydrase II (CAII) in oligodendrocyte units myelinating small and large diameter 
      fibres in the anterior medullary velum (AMV) of the adult rat (each unit 
      comprises the cell body, processes and myelin sheaths). Others have indicated 
      that myelin composition may also vary with respect to myelin basic protein (MBP) 
      and proteolipid protein (PLP), and the small (S)- and large (L)-isoforms of 
      myelin associated glycoprotein (MAG). In this study, we have determined the 
      expression of myelin proteins in oligodendrocyte unit phenotypes I-IV, which 
      myelinate fibres ranging in diameter from 0.3-12 microns diameter in the AMV, by 
      using double immunolabelling for Rip, which labels entire units, and MBP, PLP, 
      myelin oligodendrocyte glycoprotein (MOG), L-MAG and S-MAG. We show differences 
      in the expression of L- and S-MAG in units which myelinate different diameter 
      fibres: (1) type I/II units myelinating small diameter fibres had a 
      L-MAG+/S-MAG-/CAII+ phenotype; (2) type II/III units myelinating different 
      diameter fibres had a L-MAG+/S-MAG+/CAII+ phenotype; (3) type III/IV units 
      myelinated large diameter fibres had a L-MAG+/S-MAG+/CAII- phenotype. All units, 
      irrespective of fibre diameter, expressed Rip, MBP, PLP and MOG. The results 
      indicate that type I-IV units may be variants of a single oligodendrocyte 
      population and that phenotypic differences are determined by the diameter of 
      fibres within the unit. The possible significance of metabolic and biochemical 
      differences between oligodendrocytes myelinating small and large diameter axons 
      are discussed with reference to the pathology of demyelination.
FAU - Butt, A M
AU  - Butt AM
AD  - Division of Physiology, UMDS, Guy's Hospital, London, U.K.
FAU - Ibrahim, M
AU  - Ibrahim M
FAU - Gregson, N
AU  - Gregson N
FAU - Berry, M
AU  - Berry M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurocytol
JT  - Journal of neurocytology
JID - 0364620
RN  - 0 (Myelin-Associated Glycoprotein)
RN  - 0 (Proteins)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Gene Expression/physiology
MH  - Isomerism
MH  - Medulla Oblongata/*cytology
MH  - Myelin-Associated Glycoprotein/analysis/*chemistry/*genetics
MH  - Nerve Fibers, Myelinated/chemistry/enzymology
MH  - Oligodendroglia/*chemistry/*physiology
MH  - Phenotype
MH  - Proteins/analysis/genetics
MH  - Rats
MH  - Rats, Wistar
EDAT- 2000/01/20 00:00
MHDA- 2000/01/20 00:01
CRDT- 2000/01/20 00:00
PHST- 2000/01/20 00:00 [pubmed]
PHST- 2000/01/20 00:01 [medline]
PHST- 2000/01/20 00:00 [entrez]
AID - 10.1023/a:1006996713413 [doi]
PST - ppublish
SO  - J Neurocytol. 1998 Apr;27(4):271-80. doi: 10.1023/a:1006996713413.