PMID- 10640569
OWN - NLM
STAT- MEDLINE
DCOM- 20000223
LR  - 20231213
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 81
IP  - Pt 1
DP  - 2000 Jan
TI  - Evidence of synergism between African cassava mosaic virus and a new 
      double-recombinant geminivirus infecting cassava in Cameroon.
PG  - 287-97
AB  - Stem cuttings were collected in Cameroon from cassava plants displaying cassava 
      mosaic disease (CMD) symptoms. The nature of the viruses present was determined 
      by using the PCR with primers specific for the coat protein (CP) genes of African 
      cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV). All 
      samples were infected by ACMV and eight of the 50 samples were infected by both 
      ACMV and an EACMV-like virus. The complete nucleotide sequences of DNA-A and -B 
      of representative ACMV and EACMV-like viruses were determined. The DNA-A 
      component of the EACMV-like virus contained evidence of recombination in the 
      AC2-AC3 region and DNA-B also contained evidence of recombination in BC1. 
      However, both components retained gene arrangements typical of bipartite 
      begomoviruses. When Nicotiana benthamiana plants were doubly inoculated with 
      these Cameroon isolates of ACMV and EACMV (ACMV/CM, EACMV/CM) by using sap from 
      cassava plants or infectious clones, the symptoms were more severe than for 
      plants inoculated with either virus alone. Southern blot analysis of viral DNAs 
      from infected plants showed that there were significantly higher levels of 
      accumulation of both ACMV/CM components and, to a lesser extent, of EACMV/CM 
      components in mixed-infected plants than in singly infected plants. These results 
      strongly suggest the occurrence of a synergistic interaction between the two 
      viruses.
FAU - Fondong, V N
AU  - Fondong VN
AD  - International Laboratory for Tropical Agricultural Biotechnology 
      (ILTAB/IRD-DDPSC), UMSL, Molecular Electronics Bldg, 8001 Natural Bridge Rd, St 
      Louis, MO 63121-4499, USA.
FAU - Pita, J S
AU  - Pita JS
FAU - Rey, M E
AU  - Rey ME
FAU - de Kochko, A
AU  - de Kochko A
FAU - Beachy, R N
AU  - Beachy RN
FAU - Fauquet, C M
AU  - Fauquet CM
LA  - eng
SI  - GENBANK/AF112352
SI  - GENBANK/AF112353
SI  - GENBANK/AF112354
SI  - GENBANK/AF112355
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Capsid/genetics
MH  - DNA, Viral/analysis/genetics
MH  - Geminiviridae/genetics/*physiology
MH  - Manihot/*virology
MH  - Molecular Sequence Data
MH  - Mosaic Viruses/genetics/*physiology
MH  - Plant Diseases/*virology
MH  - Plants, Toxic
MH  - Polymerase Chain Reaction
MH  - Recombination, Genetic
MH  - Sequence Analysis, DNA
MH  - Nicotiana/virology
EDAT- 2000/01/21 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/21 09:00
PHST- 2000/01/21 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/21 09:00 [entrez]
AID - 10.1099/0022-1317-81-1-287 [doi]
PST - ppublish
SO  - J Gen Virol. 2000 Jan;81(Pt 1):287-97. doi: 10.1099/0022-1317-81-1-287.