PMID- 10640862
OWN - NLM
STAT- MEDLINE
DCOM- 20000329
LR  - 20171101
IS  - 0378-5866 (Print)
IS  - 0378-5866 (Linking)
VI  - 21
IP  - 6
DP  - 1999
TI  - A mechanism for acetylcholine receptor clustering distinct from agrin signaling.
PG  - 436-43
AB  - Acetylcholine receptors (AChRs) and other postsynaptic molecules cluster 
      spontaneously on cultured C2 myotubes. The frequency of clustering is enhanced by 
      neural agrin, neuraminidase, or calcium through a signaling pathway which 
      includes tyrosine phosphorylation of a muscle-specific kinase (MuSK) and the AChR 
      beta-subunit. Vicia villosa agglutinin (VVA) lectin, previously shown to 
      potentiate agrin-induced clustering on C2 myotubes, is shown here to also 
      potentiate neuraminidase- and calcium-induced clustering of AChRs, while having 
      no effect on the level of tyrosine phosphorylation of MuSK or the AChR 
      beta-subunit. We propose that VVA lectin increases the frequency of AChR 
      clustering through a mechanism that is distinct from agrin signaling, and that 
      may involve alpha-dystroglycan.
CI  - Copyright 2000 S. Karger AG, Basel
FAU - Grow, W A
AU  - Grow WA
AD  - Department of Cell Biology and Anatomy, College of Medicine, University of 
      Arizona, Tucson, AZ, USA. wgrow@online.emich.edu
FAU - Ferns, M
AU  - Ferns M
FAU - Gordon, H
AU  - Gordon H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Dev Neurosci
JT  - Developmental neuroscience
JID - 7809375
RN  - 0 (Agrin)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DAG1 protein, human)
RN  - 0 (Laminin)
RN  - 0 (Lectins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Plant Lectins)
RN  - 0 (Receptors, Cholinergic)
RN  - 0 (Vicia lectins)
RN  - 146888-27-9 (Dystroglycans)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.1 (MUSK protein, human)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 3.2.1.18 (Neuraminidase)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Agrin/*physiology
MH  - Animals
MH  - Calcium/metabolism/pharmacology
MH  - Cells, Cultured
MH  - Cytoskeletal Proteins/metabolism
MH  - Dystroglycans
MH  - Laminin/metabolism
MH  - Lectins/metabolism/pharmacology
MH  - Membrane Glycoproteins/metabolism
MH  - Mice
MH  - Muscle Fibers, Skeletal/chemistry/cytology/enzymology
MH  - N-Acetylneuraminic Acid/metabolism
MH  - Neuraminidase/pharmacology
MH  - Neuromuscular Junction/*chemistry/cytology/enzymology
MH  - Phosphorylation
MH  - *Plant Lectins
MH  - Receptor Protein-Tyrosine Kinases/metabolism
MH  - Receptors, Cholinergic/*analysis/*metabolism
MH  - Signal Transduction/*physiology
MH  - Tyrosine/metabolism
EDAT- 2000/01/21 09:00
MHDA- 2000/04/01 09:00
CRDT- 2000/01/21 09:00
PHST- 2000/01/21 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/01/21 09:00 [entrez]
AID - 17411 [pii]
AID - 10.1159/000017411 [doi]
PST - ppublish
SO  - Dev Neurosci. 1999;21(6):436-43. doi: 10.1159/000017411.