PMID- 10640999
OWN - NLM
STAT- MEDLINE
DCOM- 20000314
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 190
IP  - 1
DP  - 2000 Jan
TI  - Mast cell involvement in normal human skin wound healing: expression of monocyte 
      chemoattractant protein-1 is correlated with recruitment of mast cells which 
      synthesize interleukin-4 in vivo.
PG  - 100-6
AB  - Mast cells (MCs) are known as key cells of immediate type hypersensitivity 
      reactions. It has recently been shown that MCs regulate fibroblast proliferation 
      by heterotypic cell-cell contact and secretion of interleukin-4 (IL-4) in vitro. 
      It was therefore hypothesized that MCs may contribute to wound repair in vivo. 
      Using immunohistology and in situ hybridization, the time course of mast cell 
      recruitment and the expression of MC-attractant chemokines were analysed in a 
      human skin wound-healing model, and the production of IL-4 by MCs in vivo was 
      investigated. The data obtained indicate that the five-fold increase of the 
      tryptase+ MCs at the fibrotic border of the wound within the first 10 days is the 
      result of increased recruitment/survival of MCs or MC precursors, but not of 
      increased local proliferation. Recruitment of MCs is paralleled by the expression 
      of monocyte chemoattractant protein-1 (MCP-1), but not by other chemokines such 
      as RANTES (regulated on activation, normal T cell expressed and secreted) and/or 
      MIP (macrophage inflammatory protein)-1alpha/beta. Notably, 60-70% of MCs 
      exhibited strong and selective IL-4 immunoreactivity, whereas other resident and 
      passenger cells were rather quiescent. The data suggest that MC contribute 
      significantly to the cytokine network of wound repair via MC-derived IL-4 and 
      stimulation of fibroblast proliferation.
CI  - Copyright 2000 John Wiley & Sons, Ltd.
FAU - Trautmann, A
AU  - Trautmann A
AD  - Department of Dermatology, University of Wurzburg, Wurzburg, Germany.
FAU - Toksoy, A
AU  - Toksoy A
FAU - Engelhardt, E
AU  - Engelhardt E
FAU - Brocker, E B
AU  - Brocker EB
FAU - Gillitzer, R
AU  - Gillitzer R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Chemokine CCL2)
RN  - 0 (Inflammation Mediators)
RN  - 0 (RNA, Messenger)
RN  - 207137-56-2 (Interleukin-4)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (chymase 2)
RN  - EC 3.4.21.39 (Chymases)
RN  - EC 3.4.21.59 (Tryptases)
SB  - IM
MH  - Adult
MH  - Cell Count
MH  - Chemokine CCL2/*analysis/genetics
MH  - Chemotaxis
MH  - Chymases
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Inflammation Mediators/analysis
MH  - Interleukin-4/*analysis/genetics
MH  - Keratinocytes/metabolism
MH  - Macrophages/metabolism
MH  - Mast Cells/*metabolism/pathology
MH  - Microscopy, Interference
MH  - RNA, Messenger/analysis
MH  - Serine Endopeptidases/analysis
MH  - Skin/*metabolism/pathology
MH  - Tryptases
MH  - *Wound Healing
EDAT- 2000/01/21 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/01/21 09:00
PHST- 2000/01/21 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/01/21 09:00 [entrez]
AID - 10.1002/(SICI)1096-9896(200001)190:1<100::AID-PATH496>3.0.CO;2-Q [pii]
AID - 10.1002/(SICI)1096-9896(200001)190:1<100::AID-PATH496>3.0.CO;2-Q [doi]
PST - ppublish
SO  - J Pathol. 2000 Jan;190(1):100-6. doi: 
      10.1002/(SICI)1096-9896(200001)190:1<100::AID-PATH496>3.0.CO;2-Q.