PMID- 10641014
OWN - NLM
STAT- MEDLINE
DCOM- 20000324
LR  - 20190921
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 20
IP  - 1
DP  - 2000 Jan-Feb
TI  - Effects of phenylhydrazine or phlebotomy on peripheral blood, bone marrow and 
      erythropoietin in Wistar rats.
PG  - 25-34
AB  - This study was conducted to characterize better the response of rats to blood 
      loss and hemolysis and to incorporate automated methods into the routine 
      evaluations of those responses. Serial phlebotomies of 1.5-2.0 ml of blood per 
      day for 5 days, or intraperitoneal injection of 50 mg kg(-1) phenylhydrazine 
      (PHZ) for 3 days, were used to cause anemia associated with blood loss or 
      hemolysis, respectively. Maximum decreases in red blood counts were observed on 
      Day 3 in PHZ-treated animals (68%) and Day 4 in blood-loss animals (35%). In the 
      routine complete blood count (CBC), hemoglobin, hematocrit/hemoglobin ratio and 
      erythrocyte indices could be used to discriminate between the two treatments. 
      Free plasma hemoglobin in PHZ-treated animals resulted in marked elevations of 
      mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration 
      (MCHC) with a 2:1 hematocrit/hemoglobin ratio rather than the anticipated 3:1 
      ratio. Although both groups of animals had elevated white blood cell counts, 
      PHZ-treated animals also had monocytosis and basophilia. Reticulocyte counts were 
      more sensitive than erythropoietin (EPO) concentrations in predicting erythroid 
      changes. Maximum mean reticulocyte values were ca. 24% in serially phlebotomized 
      animals and >99% in PHZ-treated rats. Plasma EPO levels were 4-10-fold higher 
      than EPO levels in urine, kidney or liver. Flow cytometric differentials of rat 
      bone marrow using 2, 7-dichlorofluorescin successfully predicted erythroid 
      hyperplasia in both experimental groups. Erythrocyte indices returned to normal 
      within 14 days and the remaining CBC parameters were normal within 28 days for 
      both treatment groups. Reticulocyte counts remained slightly elevated on Day 28, 
      but were normal when assessed at Day 56 in blood-loss and PHZ-treated animals.
CI  - Copyright 2000 John Wiley & Sons, Ltd
FAU - Criswell, K A
AU  - Criswell KA
AD  - Pathology and Experimental Toxicology, Parke-Davis Pharmaceutical Research, 
      Warner-Lambert Co., Ann Arbor, MI 48105, USA. CRISWEK@AA.WL.COM
FAU - Sulkanen, A P
AU  - Sulkanen AP
FAU - Hochbaum, A F
AU  - Hochbaum AF
FAU - Bleavins, M R
AU  - Bleavins MR
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Phenylhydrazines)
RN  - 064F424C9K (phenylhydrazine)
RN  - 11096-26-7 (Erythropoietin)
SB  - IM
MH  - Anemia, Hemolytic/blood/*pathology
MH  - Animals
MH  - *Blood Cell Count/drug effects
MH  - *Bone Marrow Cells/drug effects
MH  - Erythrocyte Indices
MH  - Erythropoietin/*analysis/blood
MH  - Flow Cytometry
MH  - Hematologic Tests
MH  - Hemorrhage/pathology
MH  - Male
MH  - Phenylhydrazines/administration & dosage/*pharmacology
MH  - *Phlebotomy
MH  - Rats
MH  - Rats, Wistar
MH  - Reticulocyte Count
EDAT- 2000/01/21 09:00
MHDA- 2000/04/01 09:00
CRDT- 2000/01/21 09:00
PHST- 2000/01/21 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/01/21 09:00 [entrez]
AID - 10.1002/(SICI)1099-1263(200001/02)20:1<25::AID-JAT624>3.0.CO;2-7 [pii]
AID - 10.1002/(sici)1099-1263(200001/02)20:1<25::aid-jat624>3.0.co;2-7 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2000 Jan-Feb;20(1):25-34. doi: 
      10.1002/(sici)1099-1263(200001/02)20:1<25::aid-jat624>3.0.co;2-7.