PMID- 10643698
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20220227
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 43
IP  - 1
DP  - 2000 Jan
TI  - Reduction of chemokine levels and leukocyte traffic to joints by tumor necrosis 
      factor alpha blockade in patients with rheumatoid arthritis.
PG  - 38-47
AB  - OBJECTIVE: To verify the hypothesis that in rheumatoid arthritis (RA), tumor 
      necrosis factor alpha (TNFalpha) plays a critical role in regulating leukocyte 
      trafficking and chemokine levels. METHODS: Ten patients with longstanding RA 
      received a single 10 mg/kg infusion of anti-TNFalpha monoclonal antibody (cA2). 
      The articular localization of autologous granulocytes, separated in vitro and 
      labeled with 111In, was studied by analysis of gamma-camera images both before 
      and 2 weeks after treatment. At the same sequential time points, synovial biopsy 
      samples were assessed for infiltrating CD3+ T cells, CD22+ B cells, and CD68+ 
      macrophages. Synovial tissue expression of the chemokines interleukin-8 (IL-8), 
      monocyte chemotactic protein 1 (MCP-1), macrophage inflammatory protein 1alpha 
      (MIP-1alpha), MIP-1beta, Groalpha, and RANTES was also determined. Serum IL-8 and 
      MCP-1 concentrations were measured by enzyme-linked immunosorbent assay. RESULTS: 
      Anti-TNFalpha therapy in RA significantly reduced 111In-labeled granulocyte 
      migration into affected joints. There was a simultaneous and significant 
      reduction in the numbers of infiltrating synovial CD3+ T cells, CD22+ B cells, 
      and CD68+ macrophages and in the expression of IL-8 and MCP-1, with a trend 
      toward a reduction in serum concentrations of these chemokines. CONCLUSION: 
      TNFalpha blockade reduces synovial expression of the chemokines IL-8 and MCP-1 
      and diminishes inflammatory cell migration into RA joints.
FAU - Taylor, P C
AU  - Taylor PC
AD  - Kennedy Institute of Rheumatology, London, UK.
FAU - Peters, A M
AU  - Peters AM
FAU - Paleolog, E
AU  - Paleolog E
FAU - Chapman, P T
AU  - Chapman PT
FAU - Elliott, M J
AU  - Elliott MJ
FAU - McCloskey, R
AU  - McCloskey R
FAU - Feldmann, M
AU  - Feldmann M
FAU - Maini, R N
AU  - Maini RN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (CD22 protein, human)
RN  - 0 (CD3 Complex)
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Growth Substances)
RN  - 0 (Immunoglobulins, Intravenous)
RN  - 0 (Indium Radioisotopes)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-8)
RN  - 0 (Lectins)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 2)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
CIN - Arthritis Rheum. 2001 Jan;44(1):245-6. PMID: 11212171
CIN - Arthritis Rheum. 2001 Dec;44(12):2937-9. PMID: 11762957
MH  - Aged
MH  - Antibodies, Monoclonal/therapeutic use
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, B-Lymphocyte/analysis
MH  - Arthritis, Rheumatoid/diagnostic imaging/*immunology/*therapy
MH  - B-Lymphocytes/chemistry/cytology/immunology
MH  - CD3 Complex/analysis
MH  - *Cell Adhesion Molecules
MH  - Cell Movement/*immunology
MH  - Chemokine CCL2/blood/immunology
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CCL5/blood/immunology
MH  - Chemokine CXCL1
MH  - Chemokines/*blood/immunology
MH  - *Chemokines, CXC
MH  - Chemotactic Factors/blood/immunology
MH  - Female
MH  - Growth Substances/blood/immunology
MH  - Humans
MH  - Immunoglobulins, Intravenous
MH  - Indium Radioisotopes
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Interleukin-8/blood/immunology
MH  - Joints/cytology/immunology/metabolism
MH  - *Lectins
MH  - Leukocytes/chemistry/*cytology/immunology
MH  - Macrophage Inflammatory Proteins/blood/immunology
MH  - Male
MH  - Middle Aged
MH  - Neutrophils/chemistry/cytology/immunology
MH  - Radionuclide Imaging
MH  - Sialic Acid Binding Ig-like Lectin 2
MH  - Synovial Fluid/cytology/immunology/metabolism
MH  - Synovial Membrane/cytology/immunology/metabolism
MH  - T-Lymphocytes/chemistry/cytology/immunology
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors/immunology
EDAT- 2000/01/22 00:00
MHDA- 2000/01/22 00:01
CRDT- 2000/01/22 00:00
PHST- 2000/01/22 00:00 [pubmed]
PHST- 2000/01/22 00:01 [medline]
PHST- 2000/01/22 00:00 [entrez]
AID - 10.1002/1529-0131(200001)43:1<38::AID-ANR6>3.0.CO;2-L [doi]
PST - ppublish
SO  - Arthritis Rheum. 2000 Jan;43(1):38-47. doi: 
      10.1002/1529-0131(200001)43:1<38::AID-ANR6>3.0.CO;2-L.