PMID- 10644453
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20061115
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 62
IP  - 3
DP  - 1999 Dec 15
TI  - Chromosomal localization of phospholipase A2 activating protein, an Ets2 target 
      gene, to 9p21.
PG  - 529-32
AB  - A murine Ets2 target gene isolated by differential display cloning was identified 
      as the phospholipase A2 activating protein (PLAA) gene. A 2.7-kb human cDNA 
      demonstrating high homology to mouse and rat Plaa genes was then isolated and 
      characterized. Human PLAA contains six WD-40 repeat motifs and three different 
      protein kinase consensus domains. Fluorescence in situ hybridization (FISH) 
      mapping placed PLAA on chromosome 9p21, a region frequently deleted in various 
      cancers. A comprehensive mapping strategy was employed to define further the 
      chromosomal localization of PLAA relative to CDKN2A within the 9p21 locus. 
      Radiation hybrid mapping placed the gene 7.69 cR from WI-5735 (LOD >3.0), a 
      marker in close proximity to CDKN2A and CDKN2B. Yeast artificial chromosome (YAC) 
      mapping localized PLAA proximal to the CDKN2A/CDKN2B genes and to a region 
      flanked by D9S171 and INFA commonly deleted in many neoplasms. Two YACs contained 
      both PLAA and D9S259, a marker present in a second more proximal minimal deleted 
      region observed in cutaneous melanoma and squamous cell lung carcinoma. 
      Double-color fiber FISH mapping confirmed the location of PLAA centromeric to 
      D9S171 and CDKN2A/CDKN2B. The mapping data suggest a possible tumor suppressor 
      role for this gene.
CI  - Copyright 1999 Academic Press.
FAU - Beatty, B G
AU  - Beatty BG
AD  - Department of Cell and Molecular Biology, Ontario Cancer Institute, University of 
      Toronto, Toronto, Ontario, M5G 1L5, Canada. barbara@beatty5.com
FAU - Qi, S
AU  - Qi S
FAU - Pienkowska, M
AU  - Pienkowska M
FAU - Herbrick, J A
AU  - Herbrick JA
FAU - Scheidl, T
AU  - Scheidl T
FAU - Zhang, Z M
AU  - Zhang ZM
FAU - Kola, I
AU  - Kola I
FAU - Scherer, S W
AU  - Scherer SW
FAU - Seth, A
AU  - Seth A
LA  - eng
SI  - GENBANK/AF145020
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ERF protein, human)
RN  - 0 (ETS2 protein, human)
RN  - 0 (Ets2 protein, mouse)
RN  - 0 (Ets2 protein, rat)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Protein c-ets-2)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (phospholipase A2-activating protein)
SB  - IM
MH  - Animals
MH  - Bacteriophage P1/genetics
MH  - Carcinoma, Squamous Cell/genetics
MH  - Chromosomes, Artificial, Yeast/genetics
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - *DNA-Binding Proteins
MH  - Genes, Tumor Suppressor/*genetics
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Lod Score
MH  - Lung Neoplasms/genetics
MH  - Melanoma/genetics
MH  - Mice
MH  - *Physical Chromosome Mapping
MH  - Proteins/*genetics
MH  - Proto-Oncogene Protein c-ets-2
MH  - Proto-Oncogene Proteins/*physiology
MH  - Rats
MH  - *Repressor Proteins
MH  - Skin Neoplasms/genetics
MH  - Trans-Activators/*physiology
MH  - *Transcription Factors
EDAT- 2000/01/25 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/01/25 09:00
PHST- 2000/01/25 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/01/25 09:00 [entrez]
AID - S0888-7543(99)95999-2 [pii]
AID - 10.1006/geno.1999.5999 [doi]
PST - ppublish
SO  - Genomics. 1999 Dec 15;62(3):529-32. doi: 10.1006/geno.1999.5999.