PMID- 10646853
OWN - NLM
STAT- MEDLINE
DCOM- 20000204
LR  - 20051117
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 60
IP  - 1
DP  - 2000 Jan 1
TI  - Identical clonality of sporadic gastrinomas at multiple sites.
PG  - 60-3
AB  - Gastrinomas are neuroendocrine neoplasms that occur sporadically and in patients 
      with multiple endocrine neoplasia type 1 (MEN1). In MEN1, multiple gastrinomas 
      have been shown to arise by independent clonal events (Debelenko, et al., Cancer 
      Res., 57: 2238-2243, 1997). The purpose of the present study was to analyze 
      clonality in 20 sporadic gastrinomas from eight patients in whom the tumor was 
      present in at least two separate sites. A combination of methods was used to 
      assess clonality, including MEN1 gene mutation analysis, loss of heterozygosity 
      analysis of the MEN1 locus, and analysis of X-chromosome inactivation at the 
      human androgen receptor locus (human androgen receptor analysis). In three 
      patients, a somantic MEN1 gene mutation was detected in the tumor. Identical 
      mutations were found in other tumors at different sites within the same patients. 
      Human androgen receptor analysis in three informative patients and loss of 
      heterozygosity analysis in five patients revealed identical clonal patterns in 
      the tumors from multiple sites in each patient. We conclude that sporadic 
      gastrinomas at multiple sites are monoclonal and that MEN1 gene alterations in 
      gastrinomas occur before the development of tumor metastases.
FAU - Goebel, S U
AU  - Goebel SU
AD  - Digestive Diseases Branch, National Institute of Diabetes, Digestive and Kidney 
      Diseases, NIH, Bethesda, MD 20892, USA.
FAU - Vortmeyer, A O
AU  - Vortmeyer AO
FAU - Zhuang, Z
AU  - Zhuang Z
FAU - Serrano, J
AU  - Serrano J
FAU - Jensen, R T
AU  - Jensen RT
FAU - Lubensky, I A
AU  - Lubensky IA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - DNA Mutational Analysis
MH  - DNA, Neoplasm/*genetics
MH  - Dosage Compensation, Genetic
MH  - Female
MH  - Gastrinoma/*genetics
MH  - Genetic Markers
MH  - Humans
MH  - *Loss of Heterozygosity
MH  - Lymph Nodes
MH  - Male
MH  - Middle Aged
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
EDAT- 2000/01/26 00:00
MHDA- 2000/01/26 00:01
CRDT- 2000/01/26 00:00
PHST- 2000/01/26 00:00 [pubmed]
PHST- 2000/01/26 00:01 [medline]
PHST- 2000/01/26 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 2000 Jan 1;60(1):60-3.