PMID- 10648823
OWN - NLM
STAT- MEDLINE
DCOM- 20000222
LR  - 20231213
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 466
IP  - 1
DP  - 2000 Jan 21
TI  - Strongly decreased gap junctional permeability to inositol 1,4, 5-trisphosphate 
      in connexin32 deficient hepatocytes.
PG  - 112-4
AB  - Livers from connexin32 (Cx32) deficient mice have been shown to be defective in 
      hormonally induced glucose mobilization. In order to determine whether this 
      effect is due to decreased diffusion of the second messenger inositol 
      1,4,5-trisphosphate (IP(3)) between hepatocytes, we injected iontophoretically 
      different amounts of IP(3) in Fura-2 loaded hepatocyte doublets (i.e. cell pairs) 
      from wild type or Cx32 deficient mice. Whereas 84% of wild type hepatocytes 
      showed an intercellular Ca(2+) wave spreading from the injected cell to the 
      neighboring cell, only 25% of Cx32 deficient hepatocyte doublets did so. The 
      amount of IP(3) necessary to induce an intercellular Ca(2+) wave in Cx32 
      deficient hepatocyte doublets was estimated to be about 25-fold higher than in 
      wild type doublets. This confirms the notion that the low hormonally or 
      electrically induced glucose mobilization found in Cx32 deficient livers relative 
      to wild type livers is due to largely hindered diffusion of IP(3) between Cx32 
      deficient hepatocytes.
FAU - Niessen, H
AU  - Niessen H
AD  - Institut fur Genetik, Abteilung Molekulare Genetik, Universitat Bonn, 
      Romerstrasse 164, 53117, Bonn, Germany.
FAU - Willecke, K
AU  - Willecke K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Connexins)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cell Membrane Permeability
MH  - Connexins/*deficiency/genetics/metabolism
MH  - Female
MH  - Gap Junctions/*metabolism
MH  - Glucose/metabolism
MH  - In Vitro Techniques
MH  - Inositol 1,4,5-Trisphosphate/administration & dosage/*metabolism
MH  - Iontophoresis
MH  - Liver/cytology/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Second Messenger Systems
MH  - Gap Junction beta-1 Protein
EDAT- 2000/01/29 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - S0014-5793(99)01770-6 [pii]
AID - 10.1016/s0014-5793(99)01770-6 [doi]
PST - ppublish
SO  - FEBS Lett. 2000 Jan 21;466(1):112-4. doi: 10.1016/s0014-5793(99)01770-6.