PMID- 10650214
OWN - NLM
STAT- MEDLINE
DCOM- 20000307
LR  - 20181130
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 183
IP  - 1
DP  - 2000 Feb 1
TI  - Comparison of viable cell counts and fluorescence in situ hybridization using 
      specific rRNA-based probes for the quantification of human fecal bacteria.
PG  - 125-9
AB  - Conventional cultivation and fluorescence in situ hybridization (FISH) using 16S 
      rRNA-based probes were compared for the enumeration of human colonic bacteria. 
      Groups of common intestinal anaerobic bacteria were enumerated in slurries 
      prepared from fecal samples of three healthy volunteers. To introduce variation 
      between the samples, they were incubated for 48 h in batch culture (anaerobic) 
      fermenters at 37 degrees C, and pure cultures of Bifidobacterium infantis, 
      Clostridium perfringens, or Lactobacillus acidophilus were added. Samples were 
      taken from the fermenters at different times. Total anaerobes, bifidobacteria, 
      bacteroides, clostridia, and lactobacilli were enumerated by both plating and 
      FISH. The results showed that plate counts of total anaerobes, bifidobacteria, 
      lactobacilli and bacteroides were approximately ten-fold lower than the 
      corresponding FISH counts. Numbers of clostridia were higher using the plating 
      method, probably because the clostridia probe used in FISH analyses was designed 
      to only detect part of the genus Clostridium. The introduced variation in the 
      methods could be detected by both methods and was comparable.
FAU - Harmsen, H J
AU  - Harmsen HJ
AD  - Department of Medical Microbiology, University of Groningen, Hanzeplein 1, 9713 
      GZ, Groningen, The Netherlands.
FAU - Gibson, G R
AU  - Gibson GR
FAU - Elfferich, P
AU  - Elfferich P
FAU - Raangs, G C
AU  - Raangs GC
FAU - Wildeboer-Veloo, A C
AU  - Wildeboer-Veloo AC
FAU - Argaiz, A
AU  - Argaiz A
FAU - Roberfroid, M B
AU  - Roberfroid MB
FAU - Welling, G W
AU  - Welling GW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Adult
MH  - Bacteria, Anaerobic/classification/genetics/growth & development/*isolation & 
      purification
MH  - Colon/microbiology
MH  - *Colony Count, Microbial
MH  - Feces/*microbiology
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - RNA Probes
MH  - RNA, Ribosomal, 16S/*genetics
EDAT- 2000/01/29 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - S0378-1097(99)00649-7 [pii]
AID - 10.1111/j.1574-6968.2000.tb08945.x [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2000 Feb 1;183(1):125-9. doi: 
      10.1111/j.1574-6968.2000.tb08945.x.