PMID- 10651777
OWN - NLM
STAT- MEDLINE
DCOM- 20000314
LR  - 20190831
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 30
IP  - 2
DP  - 2000 Feb
TI  - Phleum pratense-specific T cells of allergic rhinitis patients display a broader 
      recognition pattern than phleum pratense-specific serum immunoglobulin E.
PG  - 242-54
AB  - BACKGROUND: The role of allergen-specific CD4+ T lymphocytes in the 
      pathophysiology of atopic disease is well established. Previous studies on 
      allergen-specific T-cell responses have focused on the recognition of single 
      major allergens to identify T-cell epitopes. OBJECTIVE: However, it is not clear 
      whether immune responses to allergen extracts are exclusively targeted at major 
      allergens or whether additional proteins are recognized. METHODS: Here we 
      describe the Phleum pratense-specific immunoglobulin E (IgE) and T-cell responses 
      of six allergic rhinitis patients. Reactivity was measured to size-separated 
      fractions of a P. pratense extract as well as to the purified major allergens Phl 
      p 1, Phl p 2/3 and Phl p 5. RESULTS: The specificity of the patients' serum IgE, 
      measured in a fluid phase assay, was restricted to one or two of the major 
      allergens. Even though the majority of the patients had IgE antibodies reactive 
      with a single major allergen, one patient reacted with both Phl p 5 and with Phl 
      p 2/3. Analysis of the T-cell repertoire with P. pratense-specific T-cell lines 
      (TCLs) and CD4+ T-cell clones (TCCs) revealed that at least six different 
      proteins were recognized, including the three major allergens, most notably Phl p 
      5. Simultaneous production of IL-5 and interferon (IFN) -gamma was detected in 
      supernatants of the TCLs stimulated with P. pratense extract and the major 
      allergens. CONCLUSION: These results indicate that allergic rhinitis patients 
      have a large pool of circulating allergen-specific CD4+ T cells that recognize 
      many different proteins in an allergenic extract, whereas only a small number of 
      these proteins are recognized by serum IgE.
FAU - Van Neerven, R J
AU  - Van Neerven RJ
AD  - ALK-Abello, Horsholm, Denmark; Current address: Tanox Pharma BV, Amsterdam, The 
      Netherlands.
FAU - Arnved, J
AU  - Arnved J
FAU - Ipsen, H
AU  - Ipsen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Plant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/immunology
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cell Line
MH  - Chromatography, Gel/methods
MH  - Cytokines/metabolism
MH  - Humans
MH  - Immunoglobulin E/blood/*immunology
MH  - Plant Proteins/*immunology
MH  - Poaceae/*immunology
MH  - Pollen/immunology
MH  - Rhinitis, Allergic, Seasonal/*immunology
EDAT- 2000/01/29 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - cea707 [pii]
AID - 10.1046/j.1365-2222.2000.00707.x [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2000 Feb;30(2):242-54. doi: 10.1046/j.1365-2222.2000.00707.x.