PMID- 10652163
OWN - NLM
STAT- MEDLINE
DCOM- 20000315
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 51
IP  - 2
DP  - 2000 Feb
TI  - Recruitment of monocyte derived dendritic cells ex vivo from SIV infected and 
      non-infected cynomolgus monkeys.
PG  - 186-94
AB  - This study shows that characteristic dendritic, antigen presenting cells, can be 
      generated from adherent peripheral blood mononuclear cells (PBMC)/monocytes of 
      uninfected and SIVsm-infected cynomolgus monkeys after stimulation in vitro with 
      granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin (IL)-4. 
      The recruitment of monocyte derived dendritic cells (MDDC) was usually possible 
      irrespective of the level of immunodeficiency (CD4-level) and viremia. The 
      cynomolgus MDDC closely resembled their human counterpart (immature MDDC) with 
      regard to capacity to upregulate CD1a, CD40, CD86 and human leukocyte antigen 
      (HLA)-DR and develop dendrites and veiled processes. Such MDDC also increased 
      their capacity for antigen uptake (dextran endocytoses/macropinocytosis) and for 
      induction of T-cell proliferation in mixed leukocyte reaction (MLR) assays. 
      However, although no clear difference with regard to phenotype and morphology was 
      seen between MDDC from SIV-infected and uninfected monkeys, a reduction in MLR 
      responsiveness in MDDC from SIV infected monkeys was consistently detected within 
      each experiment.
FAU - Soderlund, J
AU  - Soderlund J
AD  - Immunopathology Laboratory, Karolinska Institutet, Stockholm, Sweden.
FAU - Nilsson, C
AU  - Nilsson C
FAU - Ekman, M
AU  - Ekman M
FAU - Walther, L
AU  - Walther L
FAU - Gaines, H
AU  - Gaines H
FAU - Biberfeld, G
AU  - Biberfeld G
FAU - Biberfeld, P
AU  - Biberfeld P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Dextrans)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/ultrastructure/virology
MH  - Dextrans/metabolism
MH  - Endocytosis/immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Lymphocyte Culture Test, Mixed
MH  - Macaca fascicularis
MH  - Monocytes/*immunology/ultrastructure
MH  - Pinocytosis/immunology
MH  - Simian Acquired Immunodeficiency Syndrome/blood/*immunology
MH  - Simian Immunodeficiency Virus/*immunology
EDAT- 2000/01/29 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/01/29 09:00
PHST- 2000/01/29 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/01/29 09:00 [entrez]
AID - sji679 [pii]
AID - 10.1046/j.1365-3083.2000.00679.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2000 Feb;51(2):186-94. doi: 10.1046/j.1365-3083.2000.00679.x.