PMID- 10652275
OWN - NLM
STAT- MEDLINE
DCOM- 20000225
LR  - 20181113
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 14
IP  - 2
DP  - 2000 Jan 15
TI  - Dynamic organization of chromosomal DNA in Escherichia coli.
PG  - 212-23
AB  - We have revealed the subcellular localization of different DNA segments that are 
      located at approximately 230-kb intervals on the Escherichia coli chromosome 
      using fluorescence in situ hybridization (FISH). The series of chromosome 
      segments is localized within the cell in the same order as the chromosome map. 
      The large chromosome region including oriC shows similar localization patterns, 
      which we call the Ori domain. In addition, the localization pattern of the large 
      segment including dif is characteristic of the replication terminus region. The 
      segment also shows similar localization patterns, which we call the Ter domain. 
      In newborn cells, Ori and Ter domains of the chromosome are differentially 
      localized near opposite cell poles. Subsequently, in the B period, the Ori domain 
      moves toward mid-cell before the initiation of replication, and the Ter domain 
      tends to relocate at mid-cell. An inversion mutant, in which the Ter domain is 
      located close to oriC, shows abnormal subcellular localization of ori and dif 
      segments, resulting in frequent production of anucleate cells. These studies thus 
      suggest that the E. coli chromosome is organized to form a compacted ring 
      structure with the Ori and Ter domains; these domains participate in the cell 
      cycle-dependent localization of the chromosome.
FAU - Niki, H
AU  - Niki H
AD  - "Unit Process and Combined Circuit," PRESTO, Japan Science and Technology 
      Corporation (JST), Kumamoto, 862-0976, Japan. niki@gpo.kumamoto-u.ac.jp
FAU - Yamaichi, Y
AU  - Yamaichi Y
FAU - Hiraga, S
AU  - Hiraga S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Cell Division/genetics
MH  - Cell Nucleus/genetics
MH  - Chromosome Inversion
MH  - Chromosome Mapping
MH  - Chromosomes, Bacterial/*genetics
MH  - DNA Replication/genetics
MH  - DNA, Bacterial/*metabolism
MH  - Escherichia coli/cytology/*genetics
MH  - Mutation/genetics
MH  - Replication Origin/genetics
MH  - Subcellular Fractions/metabolism
PMC - PMC316355
EDAT- 2000/02/01 09:00
MHDA- 2000/03/04 09:00
PMCR- 2000/07/15
CRDT- 2000/02/01 09:00
PHST- 2000/02/01 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/02/01 09:00 [entrez]
PHST- 2000/07/15 00:00 [pmc-release]
PST - ppublish
SO  - Genes Dev. 2000 Jan 15;14(2):212-23.