PMID- 10655198
OWN - NLM
STAT- MEDLINE
DCOM- 20000327
LR  - 20190501
IS  - 0007-1161 (Print)
IS  - 1468-2079 (Electronic)
IS  - 0007-1161 (Linking)
VI  - 84
IP  - 2
DP  - 2000 Feb
TI  - Fluid transport by cultured corneal epithelial cell layers.
PG  - 199-204
AB  - BACKGROUND/AIMS: Fluid transport across the in vitro corneal epithelium is short 
      lived, hence difficult to detect and characterise. Since stable rates of fluid 
      transport across several cultured epithelial cell layers have been demonstrated, 
      the behaviour of confluent SV40 transformed rabbit corneal epithelial cells 
      (tRCEC) grown on permeable supports was examined. METHODS: Fluid transport was 
      determined with a nanoinjector volume clamp; the specific electrical resistance 
      of the layers was 184 (SEM 9) Omega cm(2). tRCEC layers transported fluid (from 
      basal to apical) against a pressure head of 3 cm H(2)O for 2-3 hours. RESULTS: In 
      the first hour, the rate of fluid transport was 5.2 (0.5) microl/h/cm(-2) (n=23), 
      which is comparable with that found in other epithelia. Fluid transport was 
      completely inhibited in 15-30 minutes by either 100 microM ouabain (n=6), 50 
      microM bumetanide (n=6), or 1 microM endothelin-1 (ET-1; n=6). Preincubation with 
      10 microM BQ123 (an ET(A) receptor antagonist) obviated inhibition by ET-1 (n=6). 
      ET-1 also caused a 22% decrease in specific resistance. CONCLUSIONS: Fluid 
      transport appears to depend on transepithelial Cl(- )transport since (1) their 
      directions are the same (stroma-->tear), and (2) both bumetanide and ouabain 
      inhibit it with similar time course. tRCEC appear useful to investigate aspects 
      of the physiology and pharmacology of fluid transport across this layer, 
      including receptor mediated control of this process.
FAU - Yang, H
AU  - Yang H
AD  - Department of Biological Sciences, SUNY College of Optometry, 100 East 24th 
      Street, New York, NY 10010-3677, USA.
FAU - Reinach, P S
AU  - Reinach PS
FAU - Koniarek, J P
AU  - Koniarek JP
FAU - Wang, Z
AU  - Wang Z
FAU - Iserovich, P
AU  - Iserovich P
FAU - Fischbarg, J
AU  - Fischbarg J
LA  - eng
GR  - EY04795/EY/NEI NIH HHS/United States
GR  - EY06178/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Br J Ophthalmol
JT  - The British journal of ophthalmology
JID - 0421041
RN  - 0 (Chlorides)
RN  - 0 (Receptors, Endothelin)
RN  - 0 (Sulfonamides)
RN  - 0Y2S3XUQ5H (Bumetanide)
RN  - 5ACL011P69 (Ouabain)
SB  - IM
MH  - Animals
MH  - Biological Transport, Active/drug effects/physiology
MH  - Body Fluids/*metabolism
MH  - Bumetanide/pharmacology
MH  - Cell Line, Transformed
MH  - Chlorides
MH  - Diffusion Chambers, Culture
MH  - Epithelium, Corneal/drug effects/*metabolism
MH  - Humans
MH  - Ouabain/pharmacology
MH  - Rabbits
MH  - Receptors, Endothelin/metabolism
MH  - Sulfonamides/pharmacology
PMC - PMC1723367
EDAT- 2000/02/03 09:00
MHDA- 2000/04/01 09:00
PMCR- 2003/02/01
CRDT- 2000/02/03 09:00
PHST- 2000/02/03 09:00 [pubmed]
PHST- 2000/04/01 09:00 [medline]
PHST- 2000/02/03 09:00 [entrez]
PHST- 2003/02/01 00:00 [pmc-release]
AID - 10.1136/bjo.84.2.199 [doi]
PST - ppublish
SO  - Br J Ophthalmol. 2000 Feb;84(2):199-204. doi: 10.1136/bjo.84.2.199.