PMID- 10656921
OWN - NLM
STAT- MEDLINE
DCOM- 20000218
LR  - 20190905
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 11
IP  - 2
DP  - 2000 Feb
TI  - Mouse hexokinase II gene: structure, cDNA, promoter analysis, and expression 
      pattern.
PG  - 91-6
AB  - In mammalian tissues, the phosphorylation of intracellular glucose to 
      glucose-6-phosphate (Glu-6-P) is facilitated by four distinct hexokinase (HK) 
      isoenzymes, designated as HKI-IV. Because of the role of HKII as a leading 
      glycolytic enzyme in insulin-sensitive tissues such as skeletal muscle, heart, 
      and adipose tissue, defects in HKII function could contribute to the development 
      of insulin resistance and perhaps Type 2 diabetes. As a first step towards 
      elucidation of the physiological role of HKII in insulin resistance and type 2 
      diabetes using mouse knock-out models, we determined the genomic structure, 
      sequence of the cDNA and of 4.8 kb of the 5' regulatory region, and 
      tissue-specific expression of the mouse HKII gene. The gene comprises 18 exons 
      that span approximately 50 kb of DNA. Nucleotide sequence of the proximal 
      promoter revealed a number of conserved putative transcription factor binding 
      motifs. We also found numerous repeat elements throughout the mouse HKII gene. 
      The mouse HKII cDNA is approximately 5.5 kb in length and contains an open 
      reading frome of 2751 bp encoding a protein of 917 amino acids. The mouse HKII 
      gene is predominantly expressed in skeletal muscle, heart, and adipose tissue. 
      The transcription initiation and polyadenylation sites for the mouse HKII mRNA 
      were similar to those of the rat and human genes.
FAU - Heikkinen, S
AU  - Heikkinen S
AD  - Department of Medicine, University of Kuopio, Finland.
FAU - Suppola, S
AU  - Suppola S
FAU - Malkki, M
AU  - Malkki M
FAU - Deeb, S S
AU  - Deeb SS
FAU - Janne, J
AU  - Janne J
FAU - Laakso, M
AU  - Laakso M
LA  - eng
SI  - GENBANK/AJ238540
SI  - GENBANK/Y11666
SI  - GENBANK/Y11667
SI  - GENBANK/Y11668
GR  - HL30086/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - *Gene Expression
MH  - Hexokinase/*genetics/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - *Promoter Regions, Genetic
MH  - Sequence Analysis, DNA
EDAT- 2000/02/05 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/02/05 09:00
PHST- 2000/02/05 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/02/05 09:00 [entrez]
AID - MG99-SB123 [pii]
AID - 10.1007/s003350010019 [doi]
PST - ppublish
SO  - Mamm Genome. 2000 Feb;11(2):91-6. doi: 10.1007/s003350010019.