PMID- 10657634
OWN - NLM
STAT- MEDLINE
DCOM- 20000309
LR  - 20190515
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 164
IP  - 4
DP  - 2000 Feb 15
TI  - Transcription factor PU.1 is necessary for development of thymic and myeloid 
      progenitor-derived dendritic cells.
PG  - 1855-61
AB  - Dendritic cells (DCs) are a heterogeneous population of cells that are 
      specialized for Ag processing and presentation. These cells are believed to 
      derive from both myeloid- and lymphoid-committed precursors. Normal human 
      PBMC-derived, human CD14+ cell (monocyte)-derived, and mouse hematopoietic 
      progenitor-derived DCs were shown to express the hematopoietic cell-restricted, 
      ets family transcription factor PU.1. These populations represent myeloid 
      progenitor-derived DCs. Hematopoietic progenitor cells from PU.1 gene-disrupted 
      (null) mice were unable to generate MHC class IIhigh, CD11c+ myeloid-derived DCs 
      in vitro. Mouse thymic DCs are proposed to be derived from a committed lymphoid 
      progenitor cell that can give rise to T cells as well as DCs. Previously, we 
      showed that CD4 and CD8 T cells developed in PU.1 null mice in a delayed manner 
      and in reduced number. We examined the thymus of 10- to 12-day-old PU.1 null mice 
      and found no evidence of DEC-205+, MIDC-8+ DCs in this tissue. Our findings 
      indicate that PU.1 regulates the development of both thymic and myeloid 
      progenitor-derived populations of DCs, and expand its known role in hematopoietic 
      development.
FAU - Anderson, K L
AU  - Anderson KL
AD  - Department of Molecular Medicine, Scripps Research Institute, La Jolla, CA 92037, 
      USA.
FAU - Perkin, H
AU  - Perkin H
FAU - Surh, C D
AU  - Surh CD
FAU - Venturini, S
AU  - Venturini S
FAU - Maki, R A
AU  - Maki RA
FAU - Torbett, B E
AU  - Torbett BE
LA  - eng
GR  - AI38385/AI/NIAID NIH HHS/United States
GR  - DK49886/DK/NIDDK NIH HHS/United States
GR  - DK549381/DK/NIDDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (proto-oncogene protein Spi-1)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/genetics/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/metabolism
MH  - Hematopoietic Stem Cells/*cytology/immunology/metabolism/pathology
MH  - Humans
MH  - Immunologic Deficiency Syndromes/genetics/metabolism/pathology
MH  - Macrophages/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Proto-Oncogene Proteins/deficiency/genetics/*physiology
MH  - Thymus Gland/*cytology/immunology/pathology
MH  - Trans-Activators/deficiency/genetics/*physiology
EDAT- 2000/02/05 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/02/05 09:00
PHST- 2000/02/05 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/02/05 09:00 [entrez]
AID - ji_v164n4p1855 [pii]
AID - 10.4049/jimmunol.164.4.1855 [doi]
PST - ppublish
SO  - J Immunol. 2000 Feb 15;164(4):1855-61. doi: 10.4049/jimmunol.164.4.1855.