PMID- 10662609
OWN - NLM
STAT- MEDLINE
DCOM- 20000308
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 267
IP  - 2
DP  - 2000 Feb 15
TI  - Emergence of porcine reproductive and respiratory syndrome virus deletion 
      mutants: correlation with the porcine antibody response to a hypervariable site 
      in the ORF 3 structural glycoprotein.
PG  - 135-40
AB  - By using porcine immune sera to select a library of phage-displayed random 
      peptides, we identified an antigenic sequence (RKASLSTS) in the C-terminus of the 
      ORF 3 structural glycoprotein of European-type porcine reproductive and 
      respiratory syndrome virus (PRRSV). Through the use of overlapping reading 
      frames, the same PRRSV genetic locus codes for the ORF 3 "RKASLSTS" sequence, and 
      a previously described ORF 4 epitope (Meulenberg, J. J. M., Van Nieuwstadt, A. 
      P., Van Essen-Zandbergen, A., and Langeveld, J. P. M., 1997, J. Virol. 71, 
      6061-6067). Sequence analysis identified naturally occurring deletion mutants at 
      this ORF 34 site. Phylogenetic analysis showed the presence of a highly accurate 
      ORF 3 molecular clock, according to which deletion mutants and nondeleted viruses 
      evolved at differing speeds. Furthermore, deletion mutants and nondeleted viruses 
      evolved as separate lineages. These distinctions suggested that deletion mutants 
      were a hitherto unrecognized subtype of European-type PRRSV. Currently, deletion 
      mutants appear to be outcompeting nondeleted viruses in the field, highlighting 
      the importance of the porcine antibody response against the minor structural 
      glycoproteins of European-type PRRSV for viral evolution.
CI  - Copyright 2000 Academic Press.
FAU - Oleksiewicz, M B
AU  - Oleksiewicz MB
AD  - Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave, DK-4771, 
      Denmark.
FAU - Botner, A
AU  - Botner A
FAU - Toft, P
AU  - Toft P
FAU - Grubbe, T
AU  - Grubbe T
FAU - Nielsen, J
AU  - Nielsen J
FAU - Kamstrup, S
AU  - Kamstrup S
FAU - Storgaard, T
AU  - Storgaard T
LA  - eng
SI  - GENBANK/AF171671
SI  - GENBANK/AF171672
SI  - GENBANK/AF171673
SI  - GENBANK/AF171674
SI  - GENBANK/AF171675
SI  - GENBANK/AF171676
SI  - GENBANK/AF171677
SI  - GENBANK/AF171678
SI  - GENBANK/AF171679
SI  - GENBANK/AF171680
SI  - GENBANK/AF171681
SI  - GENBANK/AF171682
SI  - GENBANK/AF171683
SI  - GENBANK/AF171684
SI  - GENBANK/AF171685
SI  - GENBANK/AF171686
SI  - GENBANK/AF171687
SI  - GENBANK/AF171688
SI  - GENBANK/AF171689
SI  - GENBANK/AF171690
SI  - GENBANK/AF171691
SI  - GENBANK/AF171692
SI  - GENBANK/AF171693
SI  - GENBANK/AF171694
SI  - GENBANK/AF171695
SI  - GENBANK/AF171696
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Epitopes
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Open Reading Frames/genetics/immunology
MH  - Phylogeny
MH  - Porcine respiratory and reproductive syndrome 
      virus/chemistry/*genetics/immunology
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Deletion
MH  - Sequence Homology, Amino Acid
MH  - Swine
MH  - Viral Structural Proteins/genetics/immunology
EDAT- 2000/02/09 09:00
MHDA- 2000/03/11 09:00
CRDT- 2000/02/09 09:00
PHST- 2000/02/09 09:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 2000/02/09 09:00 [entrez]
AID - S0042-6822(99)90103-4 [pii]
AID - 10.1006/viro.1999.0103 [doi]
PST - ppublish
SO  - Virology. 2000 Feb 15;267(2):135-40. doi: 10.1006/viro.1999.0103.