PMID- 10662837
OWN - NLM
STAT- MEDLINE
DCOM- 20000225
LR  - 20191023
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 20
IP  - 4
DP  - 2000 Feb 15
TI  - Rapid regulation of brain-derived neurotrophic factor mRNA within eye-specific 
      circuits during ocular dominance column formation.
PG  - 1470-83
AB  - The neurotrophin brain-derived neurotrophic factor (BDNF) has emerged as a 
      candidate retrograde signaling molecule for geniculocortical axons during the 
      formation of ocular dominance columns. Here we examined whether neuronal activity 
      can regulate BDNF mRNA in eye-specific circuits in the developing cat visual 
      system. Dark-rearing throughout the critical period for ocular dominance column 
      formation decreases levels of BDNF mRNA within primary visual cortex, whereas 
      short-term (2 d) binocular blockade of retinal activity with tetrodotoxin (TTX) 
      downregulates BDNF mRNA within the lateral geniculate nucleus (LGN) and visual 
      cortical areas. Brief (6 hr to 2 d) monocular TTX blockade during the critical 
      period and also in adulthood causes downregulation in appropriate eye-specific 
      laminae in the LGN and ocular dominance columns within primary visual cortex. 
      Monocular TTX blockade at postnatal day 23 also downregulates BDNF mRNA in a 
      periodic fashion, consistent with recent observations that ocular dominance 
      columns can be detected at these early ages by physiological methods. In 
      contrast, 10 d monocular TTX during the critical period does not cause a lasting 
      decrease in BDNF mRNA expression in columns pertaining to the treated eye, 
      consistent with the nearly complete shift in physiological response properties of 
      cortical neurons in favor of the unmanipulated eye known to result from long-term 
      monocular deprivation. These observations demonstrate that BDNF mRNA levels can 
      provide an accurate "molecular readout" of the activity levels of cortical 
      neurons and are consistent with a highly local action of BDNF in strengthening 
      and maintaining active synapses during ocular dominance column formation.
FAU - Lein, E S
AU  - Lein ES
AD  - Howard Hughes Medical Institute, Department of Molecular Biology, Berkeley, 
      California 94720, USA.
FAU - Shatz, C J
AU  - Shatz CJ
LA  - eng
GR  - R01 EY002858/EY/NEI NIH HHS/United States
GR  - R37 EY002858/EY/NEI NIH HHS/United States
GR  - EY 02858/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 4368-28-9 (Tetrodotoxin)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Cats
MH  - Darkness
MH  - Dominance, Cerebral/*genetics
MH  - *Gene Expression Regulation, Developmental/drug effects
MH  - Geniculate Bodies/metabolism/physiology
MH  - RNA, Messenger/genetics
MH  - Superior Colliculi/metabolism/physiology
MH  - Tetrodotoxin/pharmacology
MH  - *Transcription, Genetic
MH  - Vision, Binocular
MH  - Vision, Monocular
MH  - Visual Cortex/growth & development/metabolism/*physiology
MH  - Visual Pathways/growth & development/metabolism/*physiology
PMC - PMC6772351
EDAT- 2000/02/09 09:00
MHDA- 2000/03/04 09:00
PMCR- 2000/08/15
CRDT- 2000/02/09 09:00
PHST- 2000/02/09 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/02/09 09:00 [entrez]
PHST- 2000/08/15 00:00 [pmc-release]
AID - 3916 [pii]
AID - 10.1523/JNEUROSCI.20-04-01470.2000 [doi]
PST - ppublish
SO  - J Neurosci. 2000 Feb 15;20(4):1470-83. doi: 10.1523/JNEUROSCI.20-04-01470.2000.