PMID- 10663380
OWN - NLM
STAT- MEDLINE
DCOM- 20000302
LR  - 20190905
IS  - 0340-5761 (Print)
IS  - 0340-5761 (Linking)
VI  - 73
IP  - 10-11
DP  - 2000 Jan
TI  - An intra-laboratory validation of the Integrated Model for the Differentiation of 
      Skin Reactions (IMDS): discrimination between (photo)allergic and (photo)irritant 
      skin reactions in mice.
PG  - 501-9
AB  - We recently presented a modified local lymph node test which made it possible to 
      quickly and reliably differentiate between irritative and allergic skin reactions 
      with extremely simple parameters. The Integrated Model for the Differentiation of 
      Skin Reactions (IMDS) test combines measurement of cell proliferation in draining 
      lymph nodes with measurement of primary ear swelling after topical application of 
      the test substance on three consecutive days. In contrast to the 'classic' skin 
      sensitisation test in guinea-pigs the IMDS test is considerably faster and is 
      based on objective measured data, not subjective skin evaluations. Like the Local 
      Lymph Node Assay (LLNA), measurement of allergic potential in the IMDS test is 
      based on the underlying immunological mechanisms, but also considers the 
      behaviour of immune competent cells following non-specific activation by 
      irritants. In addition, the IMDS test can employ UV radiation after application 
      of the substance and, therefore, make differentiation possible between different 
      types of skin photoreaction (photoallergy and photoirritation) after both topical 
      and systemic administration. Attempts to achieve this kind of discrimination with 
      the LLNA necessitate considerably greater expenditure, as proliferation in the 
      draining lymph nodes can also be induced by moderate to extreme (photo)irritants. 
      In a previous paper in which we presented the IMDS test, we examined each type of 
      reaction in reference to one single standard; the next logical step was therefore 
      a broad-based intra-laboratory validation. An important factor in the validation 
      was the use of standards that had been thoroughly examined in both guinea pig and 
      mouse systems and were also relevant with regard to estimation of the risk for 
      humans. The data presented here show that the IMDS is a simple and reliable tool 
      for obtaining fast and reproducible assessments of potential (photo)allergic and 
      (photo)irritant skin reactions to substances.
FAU - Vohr, H W
AU  - Vohr HW
AD  - Institute of Toxicology, Bayer AG, 42096 Wuppertal, Germany.
FAU - Blumel, J
AU  - Blumel J
FAU - Blotz, A
AU  - Blotz A
FAU - Homey, B
AU  - Homey B
FAU - Ahr, H J
AU  - Ahr HJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - 0 (Allergens)
RN  - 0 (Irritants)
SB  - IM
MH  - Administration, Cutaneous
MH  - Administration, Oral
MH  - Allergens/toxicity
MH  - Animals
MH  - Cell Differentiation
MH  - Dermatitis, Irritant/*diagnosis/etiology
MH  - Dermatitis, Photoallergic/*diagnosis/etiology
MH  - Disease Models, Animal
MH  - Ear, External/drug effects
MH  - Female
MH  - Irritants/toxicity
MH  - Lymph Nodes/drug effects/*pathology
MH  - Mice
MH  - Reproducibility of Results
MH  - Skin/pathology
MH  - Skin Tests/standards
MH  - Toxicity Tests/standards
MH  - Ultraviolet Rays
EDAT- 2000/02/09 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/02/09 09:00
PHST- 2000/02/09 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/02/09 09:00 [entrez]
AID - 00730501.204 [pii]
AID - 10.1007/s002040050001 [doi]
PST - ppublish
SO  - Arch Toxicol. 2000 Jan;73(10-11):501-9. doi: 10.1007/s002040050001.