PMID- 10665665
OWN - NLM
STAT- MEDLINE
DCOM- 20000223
LR  - 20190816
IS  - 0009-9163 (Print)
IS  - 0009-9163 (Linking)
VI  - 56
IP  - 6
DP  - 1999 Dec
TI  - Prevalence of human leukocyte antigen DQA1 and DQB1 alleles in Kuwaiti Arab 
      children with type 1 diabetes mellitus.
PG  - 450-6
AB  - The prevalence of human leukocyte antigen (HLA) DQB1 and DQA1 alleles has been 
      determined in 78 Kuwaiti Arab children with insulin-dependent diabetes mellitus 
      (IDDM) and in 57 normal healthy controls with similar ethnic background. The 
      typing of HLA-DQ alleles was carried out using an allele-specific DNA-based 
      polymerase chain reaction (PCR) SSP method. DR typing was also performed in 212 
      control subjects using PCR-SSP (sequence specific primer) method. A significantly 
      higher frequency of DQB1*0201 allele was found in IDDM cases compared to the 
      controls (p<0.001). There was no significant difference in the prevalence of DQB1 
      alleles *0302, *0501, and *0602 between IDDM cases and the controls. In contrast, 
      DQB1 alleles *0301, *0402, *0502, *0602, and *0603 were represented at a somewhat 
      higher frequency in controls compared to the IDDM cohort. The frequency of DQA1 
      allele *0301, which encode for an Arg at codon 52, was significantly higher in 
      the IDDM patients compared to the controls (p<0.001). The frequency of DQA1 
      allele *0302 was also higher in IDDM cases than controls (p = 0.034) but the 
      difference was less pronounced than DQA1*0301. Amongst the Arg52 alleles, no 
      significant difference was detected in the frequency of *0401 between IDDM cases 
      and the controls and the allele *0501 was detected only in controls. For 
      non-Arg52 alleles *0103, *0104, and *0201, the differences in the two groups were 
      not significant, with the exception of allele *0104 (p = 0.024). DR3 was the most 
      common type in the Kuwaiti general population (28%) and DRB1*0301 was detected in 
      41% of the individuals with DR3 specificity. Analysis of HLA-DQBI/DQA1 haplotypes 
      from IDDM cases and controls revealed a significantly high frequency of haplotype 
      DQA1*0301/DQB1*0201 between Kuwaiti IDDM cases (49/78, 63%) and the controls 
      (8/57, 14%).
FAU - Haider, M Z
AU  - Haider MZ
AD  - Pediatrics Department, Faculty of Medicine, Kuwait University, Safat. 
      haider@hsc.kuniv.edu.kw
FAU - Shaltout, A
AU  - Shaltout A
FAU - Alsaeid, K
AU  - Alsaeid K
FAU - Qabazard, M
AU  - Qabazard M
FAU - Dorman, J
AU  - Dorman J
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Clin Genet
JT  - Clinical genetics
JID - 0253664
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Adolescent
MH  - Alleles
MH  - Arabs/genetics
MH  - Child
MH  - Child, Preschool
MH  - Diabetes Mellitus, Type 1/epidemiology/*genetics/immunology
MH  - Gene Frequency/immunology
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DR Antigens/genetics
MH  - Haplotypes/immunology
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Kuwait/epidemiology
MH  - Polymerase Chain Reaction
MH  - Prevalence
EDAT- 2000/02/09 09:00
MHDA- 2000/02/26 09:00
CRDT- 2000/02/09 09:00
PHST- 2000/02/09 09:00 [pubmed]
PHST- 2000/02/26 09:00 [medline]
PHST- 2000/02/09 09:00 [entrez]
AID - 10.1034/j.1399-0004.1999.560608.x [doi]
PST - ppublish
SO  - Clin Genet. 1999 Dec;56(6):450-6. doi: 10.1034/j.1399-0004.1999.560608.x.