PMID- 10666252
OWN - NLM
STAT- MEDLINE
DCOM- 20001103
LR  - 20231213
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 5
DP  - 2000 Mar
TI  - Interactions of viral protein 3CD and poly(rC) binding protein with the 5' 
      untranslated region of the poliovirus genome.
PG  - 2219-26
AB  - The poly(rC) binding protein (PCBP) is a cellular protein required for poliovirus 
      replication. PCBP specifically interacts with two domains of the poliovirus 5' 
      untranslated region (5'UTR), the 5' cloverleaf structure, and the stem-loop IV of 
      the internal ribosome entry site (IRES). Using footprinting analysis and 
      site-directed mutagenesis, we have mapped the RNA binding site for this cellular 
      protein within the stem-loop IV domain. A C-rich sequence in a loop at the top of 
      this large domain is required for PCBP binding and is crucial for viral 
      translation. PCBP binds to stem-loop IV RNA with six-times-higher affinity than 
      to the 5' cloverleaf structure. However, the binding of the viral protein 3CD 
      (precursor of the viral protease 3C and the viral polymerase 3D) to the 
      cloverleaf RNA dramatically increases the affinity of PCBP for this RNA element. 
      The viral protein 3CD binds to the cloverleaf RNA but does not interact directly 
      with stem-loop IV nor with other RNA elements of the viral IRES. Our results 
      indicate that the interactions of PCBP with the poliovirus 5'UTR are modulated by 
      the viral protein 3CD.
FAU - Gamarnik, A V
AU  - Gamarnik AV
AD  - Department of Microbiology and Immunology, University of California, San 
      Francisco, California 94143-0414, USA.
FAU - Andino, R
AU  - Andino R
LA  - eng
GR  - N01AI40085/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoproteins)
RN  - 0 (RNA, Viral)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Viral Proteins)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.28 (3C Viral Proteases)
SB  - IM
MH  - 3C Viral Proteases
MH  - 5' Untranslated Regions/*metabolism
MH  - Base Sequence
MH  - Binding Sites
MH  - Cysteine Endopeptidases/genetics/*metabolism
MH  - *DNA-Binding Proteins
MH  - *Genome, Viral
MH  - HeLa Cells
MH  - *Heterogeneous-Nuclear Ribonucleoproteins
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Nucleic Acid Conformation
MH  - Poliovirus/genetics/*metabolism
MH  - Protein Binding/drug effects
MH  - Protein Biosynthesis
MH  - RNA, Viral/chemistry/metabolism
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - *Transcription Factors
MH  - *Viral Proteins
MH  - Virus Replication
PMC - PMC111703
EDAT- 2000/02/09 09:00
MHDA- 2001/02/28 10:01
PMCR- 2000/03/01
CRDT- 2000/02/09 09:00
PHST- 2000/02/09 09:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/02/09 09:00 [entrez]
PHST- 2000/03/01 00:00 [pmc-release]
AID - 1153 [pii]
AID - 10.1128/jvi.74.5.2219-2226.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Mar;74(5):2219-26. doi: 10.1128/jvi.74.5.2219-2226.2000.