PMID- 10666582
OWN - NLM
STAT- MEDLINE
DCOM- 20000317
LR  - 20190516
IS  - 0907-4449 (Print)
IS  - 0907-4449 (Linking)
VI  - 55
IP  - Pt 12
DP  - 1999 Dec
TI  - Crystallization and preliminary X-ray analysis of birch-pollen allergen Bet v 1 
      in complex with a murine monoclonal IgG Fab' fragment.
PG  - 2035-6
AB  - The human type I allergic response is characterized by the presence of 
      allergen-specific serum immunoglobulin E (IgE). Allergen-mediated cross-linking 
      of receptor-bound IgE on the surface of mast cells and circulating basophils 
      triggers the release of mediators, resulting in the development of the clinical 
      symptoms of allergy. In order to study the structural basis of allergen-antibody 
      interaction, a complex between the major birch-pollen allergen Bet v 1 and a Fab' 
      fragment isolated from the murine monoclonal Bet v 1 antibody BV16 has been 
      crystallized. Complex crystals belong to space group P1, with unit-cell 
      parameters a = 91.65, b = 99.14, c = 108.90 A, alpha = 105.7, beta = 98.32, gamma 
      = 97.62 degrees, and diffract to 2.9 A resolution when analyzed at 100 K using 
      synchrotron-generated X--rays.
FAU - Spangfort, M D
AU  - Spangfort MD
AD  - Biochemical Allergy Research, ALK-Abello, Boge Alle 6-8, DK-2970 Horsholm, 
      Denmark. michaels@inet.uni2.dk
FAU - Mirza, O
AU  - Mirza O
FAU - Svensson, L A
AU  - Svensson LA
FAU - Larsen, J N
AU  - Larsen JN
FAU - Gajhede, M
AU  - Gajhede M
FAU - Ipsen, H
AU  - Ipsen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Acta Crystallogr D Biol Crystallogr
JT  - Acta crystallographica. Section D, Biological crystallography
JID - 9305878
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Plant)
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Plant Proteins)
RN  - 126161-14-6 (Bet v 1 allergen, Betula)
SB  - IM
MH  - Allergens/*chemistry/*isolation & purification
MH  - Animals
MH  - Antibodies, Monoclonal/chemistry
MH  - Antigen-Antibody Reactions
MH  - Antigens, Plant
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Humans
MH  - Immunoglobulin Fab Fragments/chemistry
MH  - Immunoglobulin G/chemistry
MH  - Mice
MH  - Plant Proteins/*chemistry/*isolation & purification
MH  - Pollen/*chemistry/immunology
MH  - Rhinitis, Allergic, Seasonal/immunology
EDAT- 2000/02/10 09:00
MHDA- 2000/03/25 09:00
CRDT- 2000/02/10 09:00
PHST- 2000/02/10 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 2000/02/10 09:00 [entrez]
AID - GR0943 [pii]
AID - 10.1107/s0907444999011804 [doi]
PST - ppublish
SO  - Acta Crystallogr D Biol Crystallogr. 1999 Dec;55(Pt 12):2035-6. doi: 
      10.1107/s0907444999011804.