PMID- 10669011
OWN - NLM
STAT- MEDLINE
DCOM- 20000314
LR  - 20190822
IS  - 0041-0101 (Print)
IS  - 0041-0101 (Linking)
VI  - 38
IP  - 1
DP  - 2000 Jan
TI  - Some toxic and enzymatic activities of Bothrops ammodytoides (yarara nata) venom.
PG  - 49-61
AB  - Bothrops ammodytoides, the smallest representative of this genus, is found only 
      in Argentina. Venom was extracted from thirty adult specimens (35-70 cm in 
      length, 90-300 g in weight) captured in the Province of Buenos Aires and kept in 
      captivity. Venom yield was 3-30 mg. SDS-PAGE showed strong bands at 14.0; 23-25; 
      45; 54 and 63 kDa and weak bands at 17.0; 30.0; 40.0 and 85.0 kDa. Toxic 
      activities were: LD50 (intravenous, mice) 0.5+/-0.2 microg/g; minimal 
      procoagulant dose on human plasma (MPD-P) 35+/-2 mg/l; and minimal 
      defibrinogenating dose (MDD, mice) 6-12 microg. Hemorrhagic and/or necrotic 
      activities appear to play a major role in lethality; minimal hemorrhagic dose 
      (MHD, mice) is 10+/-2 microg/g and minimal necrotizing dose (MND, mice) is 38+/-5 
      microg. The LD50, MPD-P and MND are among the lowest in venoms from Bothrops 
      species found in Argentina. B. ammodytoides venom exhibited high proteolytic and 
      phospholipase A2 activities. Most of the B. ammodytoides venom components 
      cross-react with Bivalent Bothropic antivenom (Instituto Nacional de Produccion 
      de Biologicos ANLIS Dr. G. Malbrin, against B. alternatus and B. neuwiedii 
      venoms). One ml of antivenom neutralizes 1.2 mg of B. ammodytoides venom.
FAU - de Roodt, A R
AU  - de Roodt AR
AD  - Instituto Nacional de Produccion de Biologicos, ANLIS Dr. Carlos G. Malbran, 
      Buenos Aires, Argentina.
FAU - Dolab, J A
AU  - Dolab JA
FAU - Hajos, S E
AU  - Hajos SE
FAU - Gould, E
AU  - Gould E
FAU - Dinapoli, H
AU  - Dinapoli H
FAU - Troiano, J C
AU  - Troiano JC
FAU - Gould, J
AU  - Gould J
FAU - Dokmetjian, J C
AU  - Dokmetjian JC
FAU - Carfagnini, J C
AU  - Carfagnini JC
FAU - Fernandez, T
AU  - Fernandez T
FAU - Amoroso, M
AU  - Amoroso M
FAU - Segre, L
AU  - Segre L
FAU - Vidal, J C
AU  - Vidal JC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Antivenins)
RN  - 0 (Crotalid Venoms)
SB  - IM
MH  - Animals
MH  - Antivenins/pharmacology
MH  - Blood Coagulation/drug effects
MH  - Blotting, Western
MH  - Bothrops/*physiology
MH  - Chromatography, Gel
MH  - Crotalid Venoms/*enzymology/*toxicity
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Erythrocytes/drug effects
MH  - Hemolysis/drug effects
MH  - Hemorrhage/chemically induced/pathology
MH  - Immunochemistry
MH  - Lethal Dose 50
MH  - Mice
MH  - Muscle, Skeletal/pathology
MH  - Snake Bites/pathology
EDAT- 2000/02/11 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/02/11 09:00
PHST- 2000/02/11 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/02/11 09:00 [entrez]
AID - S0041-0101(99)00126-9 [pii]
AID - 10.1016/s0041-0101(99)00126-9 [doi]
PST - ppublish
SO  - Toxicon. 2000 Jan;38(1):49-61. doi: 10.1016/s0041-0101(99)00126-9.