PMID- 10669396
OWN - NLM
STAT- MEDLINE
DCOM- 20000411
LR  - 20191218
IS  - 0885-4513 (Print)
IS  - 0885-4513 (Linking)
VI  - 31
IP  - 1
DP  - 2000 Feb
TI  - Bioconversion of D-galactose into D-tagatose by expression of L-arabinose 
      isomerase.
PG  - 1-4
AB  - D-Tagatose is a potential bulking agent in food as a non-calorific sweetener. To 
      produce D-tagatose from cheaper resources, plasmids harbouring the L-arabinose 
      isomerase gene (araA) from Escherichia coli, Bacillus subtilis and Salmonella 
      typhimurium were constructed because L-arabinose isomerase was suggested 
      previously as an enzyme that mediates the bioconversion of galactose into 
      tagatose as well as that of arabinose to ribulose. The constructed plasmids were 
      named pTC101, pTC105 and pTC106, containing araA from E. coli, B. subtilis and S. 
      typhimurium respectively. In the cultures of recombinant E. coli with pTC101, 
      pTC105 and pTC106, tagatose was produced from galactose in 9.9, 7.1 and 6.9% 
      yields respectively. The enzyme extract of E. coli with the plasmid pTC101 also 
      converted galactose into tagatose with a 96.4% yield.
FAU - Roh, H J
AU  - Roh HJ
AD  - R&D Center, Tong Yang Confectionery Co., 30-10 Munbai-dong, Yongsan-ku, Seoul 
      140-715, Korea.
FAU - Kim, P
AU  - Kim P
FAU - Park, Y C
AU  - Park YC
FAU - Choi, J H
AU  - Choi JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Appl Biochem
JT  - Biotechnology and applied biochemistry
JID - 8609465
RN  - 0 (Hexoses)
RN  - 0 (Recombinant Proteins)
RN  - EC 5.3.1.- (Aldose-Ketose Isomerases)
RN  - EC 5.3.1.4 (L-arabinose isomerase)
RN  - T7A20Y888Y (tagatose)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Aldose-Ketose Isomerases/*genetics/*metabolism
MH  - Bacillus subtilis/genetics
MH  - Biotechnology/methods
MH  - Escherichia coli/*genetics
MH  - Galactose/*metabolism
MH  - Gene Expression Regulation, Bacterial
MH  - Genetic Engineering/*methods
MH  - Hexoses/*biosynthesis
MH  - Plasmids/genetics
MH  - Recombinant Proteins/genetics/metabolism
MH  - Salmonella typhimurium/genetics
MH  - Transformation, Bacterial/genetics
EDAT- 2000/02/11 09:00
MHDA- 2000/04/15 09:00
CRDT- 2000/02/11 09:00
PHST- 2000/02/11 09:00 [pubmed]
PHST- 2000/04/15 09:00 [medline]
PHST- 2000/02/11 09:00 [entrez]
AID - 10.1042/ba19990065 [doi]
PST - ppublish
SO  - Biotechnol Appl Biochem. 2000 Feb;31(1):1-4. doi: 10.1042/ba19990065.