PMID- 10670737
OWN - NLM
STAT- MEDLINE
DCOM- 20000225
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 735
IP  - 2
DP  - 1999 Dec 10
TI  - Routine analysis of amphetamine class drugs as their naphthaquinone derivatives 
      in human urine by high-performance liquid chromatography.
PG  - 229-41
AB  - We describe a simple HPLC method which is suitable for the routine confirmation 
      of immunoassay positive amphetamine urine samples. The precolumn derivisation 
      method employing sodium naphthaquinone-4-sulphonate was found to have adequate 
      sensitivity, selectivity and precision for the measurement of amphetamine, 
      methamphetamine, 3,4-methylenedioxymethamphetamine (MDMA), 
      3,4-methylenedioxyamphetamine (MDA), and 3,4-methylenedioxyethylamphetamine 
      (MDEA) at 500 microg/l cutoff level for confirmatory analysis of amphetamines in 
      urine. The specificity of the method is enhanced by detecting the peaks at two 
      different wavelengths. The ratios of the peak heights measured at the two 
      wavelengths were different for each of the 5 amphetamines analysed. There was no 
      interference from other phenylethylamine analogues that are commonly found in 
      "over the counter" preparations. The HPLC method is compared to a commercial TLC 
      system for detecting amphetamines in urine of drug abusers attending drug 
      rehabilitation programmes. The HPLC confirmatory method described is a viable 
      alternative to GC or to the more complex and costly GC-MS techniques for 
      confirming amphetamine, methamphetamine, MDMA, MDA and MDEA in urine of drug 
      abusers especially when used in a clinical care setting.
FAU - Talwar, D
AU  - Talwar D
AD  - Department of Clinical Biochemistry, Royal Infirmary, Glasgow, UK.
FAU - Watson, I D
AU  - Watson ID
FAU - Stewart, M J
AU  - Stewart MJ
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Amphetamines)
RN  - 0 (Naphthoquinones)
SB  - IM
MH  - Amphetamines/*urine
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Naphthoquinones/*urine
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrophotometry, Ultraviolet
EDAT- 2000/02/12 00:00
MHDA- 2000/02/12 00:01
CRDT- 2000/02/12 00:00
PHST- 2000/02/12 00:00 [pubmed]
PHST- 2000/02/12 00:01 [medline]
PHST- 2000/02/12 00:00 [entrez]
AID - 10.1016/s0378-4347(99)00426-0 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1999 Dec 10;735(2):229-41. doi: 
      10.1016/s0378-4347(99)00426-0.