PMID- 10670838
OWN - NLM
STAT- MEDLINE
DCOM- 20000301
LR  - 20190915
IS  - 1073-2322 (Print)
IS  - 1073-2322 (Linking)
VI  - 13
IP  - 2
DP  - 2000 Feb
TI  - Altered calcium regulation and function of human neutrophils during multiple 
      trauma.
PG  - 92-9
AB  - Altered intracellular Ca2+ concentration is a pivotal regulatory mechanism of 
      leukocyte function. Since polymorphonuclear neutrophils (PMN) are involved in 
      traumatic organ dysfunction, we prospectively investigated Ca2+ regulation and 
      function of circulating PMN multiple trauma patients (Group A: ISS < 27; Group B: 
      ISS > or = 27). Circulating PMN were isolated during 12 days, followed by 
      determination of formyl-methionyl-leucyl-phenylalanine (fMLP)-induced 
      PMN-superoxide production (PMN-SOP) by SOD-inhibitable ferricytochrome C 
      reduction, and PMN cytosolic Ca2+ concentration ([Ca2+]i) by fluorescent fura2/AM 
      (340/380 ratio). PMN-SOP was significantly higher in Group B (mean ISS: 39.9 +/- 
      2; n = 21) at day of admission than in controls and Group A (mean ISS: 18.2 +/- 
      1; n = 22) (P< 0.05). In Group B, the significant rise of basal [Ca2+]i between 
      Day 2 and Day 4 was associated with significant lower PMN-SOP during that period 
      (P < 0.05). The fMLP-induced [Ca2+]i response was supranormal in both groups. 
      PMN-elastase concentrations were substantially higher in Group B compared with 
      Group A until Day 4. Circulating IL-6, IL-8, and soluble TNF-receptor (55 kD) 
      were significantly increased in Group B compared with Group A at the day of 
      trauma (P < 0.05). Severe trauma is characterized by a biphasic pattern of 
      neutrophil priming characterized by early increase and secondary suppression. The 
      association of depressed neutrophil superoxide production (deactivation) and 
      elevated basal [Ca2+]i suggests Ca2+-mediated disturbance of neutrophil 
      NADPH-oxidase metabolism.
FAU - Rose, S
AU  - Rose S
AD  - University of Saarland, Department of Trauma, Hand and Reconstructive Surgery, 
      Homburg/Saar, Germany.
FAU - Illerhaus, M
AU  - Illerhaus M
FAU - Wiercinski, A
AU  - Wiercinski A
FAU - Mutschler, W
AU  - Mutschler W
FAU - Marzi, I
AU  - Marzi I
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Shock
JT  - Shock (Augusta, Ga.)
JID - 9421564
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipid Peroxides)
RN  - 11062-77-4 (Superoxides)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 3.4.21.37 (Leukocyte Elastase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adult
MH  - Calcium/*metabolism
MH  - Cytosol/metabolism
MH  - Female
MH  - Glutathione Peroxidase/blood
MH  - Humans
MH  - Incidence
MH  - Inflammation Mediators/metabolism
MH  - Injury Severity Score
MH  - Leukocyte Elastase/blood
MH  - Lipid Peroxides/blood
MH  - Male
MH  - Malondialdehyde/blood
MH  - Multiple Organ Failure/epidemiology/immunology
MH  - Multiple Trauma/*immunology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophil Activation/drug effects
MH  - Neutrophils/drug effects/enzymology/*immunology/*metabolism
MH  - Peroxidase/metabolism
MH  - Superoxides/metabolism
EDAT- 2000/02/12 09:00
MHDA- 2000/03/04 09:00
CRDT- 2000/02/12 09:00
PHST- 2000/02/12 09:00 [pubmed]
PHST- 2000/03/04 09:00 [medline]
PHST- 2000/02/12 09:00 [entrez]
AID - 10.1097/00024382-200013020-00002 [doi]
PST - ppublish
SO  - Shock. 2000 Feb;13(2):92-9. doi: 10.1097/00024382-200013020-00002.