PMID- 10671186
OWN - NLM
STAT- MEDLINE
DCOM- 20000209
LR  - 20200724
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 64
IP  - 4
DP  - 1998 Apr
TI  - Reductive dechlorination of tetrachloroethene to ethene by a two-component enzyme 
      pathway.
PG  - 1270-5
AB  - Two membrane-bound, reductive dehalogenases that constitute a novel pathway for 
      complete dechlorination of tetrachloroethene (perchloroethylene [PCE]) to ethene 
      were partially purified from an anaerobic microbial enrichment culture containing 
      Dehalococcoides ethenogenes 195. When titanium (III) citrate and methyl viologen 
      were used as reductants, PCE-reductive dehalogenase (PCE-RDase) (51 kDa) 
      dechlorinated PCE to trichloroethene (TCE) at a rate of 20 micromol/min/mg of 
      protein. TCE-reductive dehalogenase (TCE-RDase) (61 kDa) dechlorinated TCE to 
      ethene. TCE, cis-1,2-dichloroethene, and 1,1-dichloroethene were dechlorinated at 
      similar rates, 8 to 12 micromol/min/mg of protein. Vinyl chloride and 
      trans-1,2-dichloroethene were degraded at rates which were approximately 2 orders 
      of magnitude lower. The light-reversible inhibition of TCE-RDase by iodopropane 
      and the light-reversible inhibition of PCE-RDase by iodoethane suggest that both 
      of these dehalogenases contain Co(I) corrinoid cofactors. Isolation and 
      characterization of these novel bacterial enzymes provided further insight into 
      the catalytic mechanisms of biological reductive dehalogenation.
FAU - Magnuson, J K
AU  - Magnuson JK
AD  - USAF Armstrong Laboratory, Tyndall Air Force Base, Florida 32403-5323, USA. 
      jkmagnus@c-mail.apgea.army.mil
FAU - Stern, R V
AU  - Stern RV
FAU - Gossett, J M
AU  - Gossett JM
FAU - Zinder, S H
AU  - Zinder SH
FAU - Burris, D R
AU  - Burris DR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Environmental Pollutants)
RN  - 290YE8AR51 (Trichloroethylene)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.- (tetrachloroethene dehalogenase)
RN  - EC 3.- (Hydrolases)
RN  - TJ904HH8SN (Tetrachloroethylene)
SB  - IM
MH  - Environmental Pollutants/metabolism
MH  - Gram-Negative Bacteria/*enzymology
MH  - Hydrolases/chemistry/isolation & purification/*metabolism
MH  - Oxidation-Reduction
MH  - Oxidoreductases/chemistry/isolation & purification/*metabolism
MH  - Tetrachloroethylene/metabolism
MH  - Trichloroethylene/metabolism
PMC - PMC106140
EDAT- 2000/02/12 09:00
MHDA- 2000/02/19 09:00
PMCR- 1998/04/01
CRDT- 2000/02/12 09:00
PHST- 2000/02/12 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 2000/02/12 09:00 [entrez]
PHST- 1998/04/01 00:00 [pmc-release]
AID - 1515 [pii]
AID - 10.1128/AEM.64.4.1270-1275.1998 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1998 Apr;64(4):1270-5. doi: 
      10.1128/AEM.64.4.1270-1275.1998.