PMID- 10671210
OWN - NLM
STAT- MEDLINE
DCOM- 20000314
LR  - 20220317
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 30
IP  - 2
DP  - 2000 Feb
TI  - Acellular components of Chlamydia pneumoniae stimulate cytokine production in 
      human blood mononuclear cells.
PG  - 541-9
AB  - Accumulating evidence suggest that infection with Chlamydia pneumoniae is 
      associated with atherosclerosis, but the mechanisms involved remain unclear. 
      Inflammation is important in the initial phase of atherogenesis, and cytokines 
      are important in the initiation and progression of inflammation. The aim of this 
      study was to assess the capacity of acellular components of C. pneumoniae to 
      stimulate the production of pro-inflammatory cytokines and chemokines. Peripheral 
      blood mononuclear cells were stimulated in vitro with sonicated C. pneumoniae. 
      Significant amounts of TNF-alpha, IL-1, IL-6, IL-8, monocyte chemoattractant 
      protein-1 (MCP-1) and macrophage inflammatory protein-1alpha (MIP-1alpha) were 
      produced. Inhibition of endotoxin using polymyxin B revealed that chlamydial 
      endotoxin plays a minor role in the cytokine induction. Neutralization of TNF by 
      TNF-binding protein and blockade of IL-1 receptors by IL-1 receptor antagonist 
      revealed that TNF, IL-1 and IL-6 production was independent from each other, 
      whereas IL-8 synthesis was strongly dependent on endogenous TNF and IL-1. In 
      contrast, synthesis of MCP-1 and MIP-1alpha was dependent on endogenous TNF, but 
      not IL-1. In conclusion, acellular components of C. pneumoniae are a potent 
      stimulus for cytokine production, and this mechanism may have an important role 
      in the inflammatory aspects of atherogenesis.
FAU - Netea, M G
AU  - Netea MG
AD  - Department of Medicine, University Hospital Nijmegen, Nijmegen, The Netherlands. 
      M.Netea@aig.azn.nl
FAU - Selzman, C H
AU  - Selzman CH
FAU - Kullberg, B J
AU  - Kullberg BJ
FAU - Galama, J M
AU  - Galama JM
FAU - Weinberg, A
AU  - Weinberg A
FAU - Stalenhoef, A F
AU  - Stalenhoef AF
FAU - Van der Meer, J W
AU  - Van der Meer JW
FAU - Dinarello, C A
AU  - Dinarello CA
LA  - eng
GR  - AI-15614/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Cytokines)
SB  - IM
MH  - Cells, Cultured
MH  - Chlamydia Infections/*immunology
MH  - Chlamydophila pneumoniae/*immunology
MH  - Cytokines/biosynthesis/*immunology
MH  - Humans
MH  - Leukocytes, Mononuclear/*immunology/microbiology
EDAT- 2000/02/12 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/02/12 09:00
PHST- 2000/02/12 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/02/12 09:00 [entrez]
AID - 10.1002/(SICI)1521-4141(200002)30:02<541::AID-IMMU541>3.0.CO;2-K [pii]
AID - 10.1002/1521-4141(200002)30:2<541::AID-IMMU541>3.0.CO;2-X [doi]
PST - ppublish
SO  - Eur J Immunol. 2000 Feb;30(2):541-9. doi: 
      10.1002/1521-4141(200002)30:2<541::AID-IMMU541>3.0.CO;2-X.