PMID- 10673548
OWN - NLM
STAT- MEDLINE
DCOM- 20000418
LR  - 20190513
IS  - 0022-3751 (Print)
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Linking)
VI  - 523 Pt 1
IP  - Pt 1
DP  - 2000 Feb 15
TI  - Mechanically gated channel activity in cytoskeleton-deficient plasma membrane 
      blebs and vesicles from Xenopus oocytes.
PG  - 117-30
AB  - 1. A novel technique involving hypertonic stress causes membrane 'blebbing' of 
      the Xenopus oocyte and the shedding of plasma membrane vesicles (PMVs). 2. 
      Confocal fluorescence microscopy, immunocytochemistry and electron microscopy 
      indicate that blebs and PMVs lack cortical cytoskeleton and are deficient in 
      cytoskeleton proteins and devoid of microvilli. 3. Patch recordings from PMVs 
      consistently reveal mechanically gated (MG) channel activity. The MG channels 
      display the same single-channel conductance as control recordings but differ in 
      terms of reduced mechanosensitivity and adaptation to sustained stimulation. 4. 
      Whole PMV recordings show rapid and reversible activation of mechanosensitive 
      currents in response to pressure pulses. The maximal currents activated in PMVs 
      are consistent with MG channel activity recorded in patches. 5. The discrepancy 
      between MG channel activity recorded in whole PMVs and oocytes most probably 
      reflects their different membrane geometry and ability to develop activating 
      bilayer tensions. 6. We propose that membrane blebbing, which is known to occur 
      under specific physiological and pathological conditions (e.g. mitosis and 
      apoptosis), may increase mechanosensitivity independently of the intrinsic 
      properties of membrane proteins.
FAU - Zhang, Y
AU  - Zhang Y
AD  - Physiology and Biophysics and Pathology, University of Texas Medical Branch, 
      Galveston, TX 77555-0641, USA.
FAU - Gao, F
AU  - Gao F
FAU - Popov, V L
AU  - Popov VL
FAU - Wen, J W
AU  - Wen JW
FAU - Hamill, O P
AU  - Hamill OP
LA  - eng
GR  - R01-AR42782/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Hypertonic Solutions)
RN  - 0 (Ion Channels)
SB  - IM
MH  - Animals
MH  - Cell Membrane/physiology
MH  - Cytoskeleton/*physiology
MH  - Electric Conductivity
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Hypertonic Solutions/pharmacology
MH  - *Ion Channel Gating
MH  - Ion Channels/*physiology
MH  - Microscopy, Confocal
MH  - Oocytes/drug effects/*physiology
MH  - Xenopus laevis/*physiology
PMC - PMC2269789
EDAT- 2000/02/16 09:00
MHDA- 2000/04/25 09:00
PMCR- 2001/02/15
CRDT- 2000/02/16 09:00
PHST- 2000/02/16 09:00 [pubmed]
PHST- 2000/04/25 09:00 [medline]
PHST- 2000/02/16 09:00 [entrez]
PHST- 2001/02/15 00:00 [pmc-release]
AID - PHY_9616 [pii]
AID - 10.1111/j.1469-7793.2000.t01-1-00117.x [doi]
PST - ppublish
SO  - J Physiol. 2000 Feb 15;523 Pt 1(Pt 1):117-30. doi: 
      10.1111/j.1469-7793.2000.t01-1-00117.x.