PMID- 10674384
OWN - NLM
STAT- MEDLINE
DCOM- 20000314
LR  - 20191103
IS  - 1087-0024 (Print)
IS  - 1087-0024 (Linking)
VI  - 4
IP  - 3
DP  - 1999 Dec
TI  - In vitro main pathways of steroid action in cultured hair follicle cells: 
      vascular approach.
PG  - 290-5
AB  - The known role of steroids on the hair follicle leads us to investigate their 
      effects on hair follicle cell angiogenic responses in vitro. We verified, using 
      the immunohistochemical technique, whether human occipital scalp follicle cells 
      express steroid receptors in vitro. We showed that androgen and estrogen 
      receptors were expressed by dermal papilla cells (DPC) and keratinocytes from the 
      outer root sheath in vitro. With regard to steroidal enzymes (type I and II 
      5alpha-reductases and Cytochrome-p-450-aromatase), the type I 5alpha-reductase 
      gene is much more expressed in DPC than in dermal fibroblasts; however, the type 
      II 5a-reductase gene is transcribed more in dermal fibroblasts than in DPC. The 
      transcription of the two 5alpha-reductase isoform genes in cultured DPC is 
      regulated by a 5alpha-reductase inhibitor. We also demonstrated that DPC, dermal 
      fibroblasts, and outer root shealth keratinocytes expressed 
      cytochrome-p-450-aromatase. Using ELISA and reverse transcriptase-polymerase 
      chain reaction, we investigated the role played by some steroids (estrogens, 
      androgens, antiandrogens) in the modulation of vascular endothelial growth factor 
      (VEGF) expression by DPC. The association of different treatments of DPC 
      (5alpha-reductase inhibitor and androgen receptor antagonist) shows a great 
      stimulation of VEGF and aromatase expression. Strong stimulation of VEGF protein 
      and gene expression is observed in the presence of 17beta-estradiol. Also, the 
      concentration-dependent inhibition of VEGF expression by DPC using the 
      cytochrome-p-450-aromatase inhibitor, confirms the involvement of this estrogenic 
      pathway in the regulation of VEGF expression in vitro.
FAU - Lachgar, S
AU  - Lachgar S
AD  - Laboratoire de Biologie Cellulaire Cutanee, Institut de Recherche Pierre Fabre, 
      Faculte de Medecine Rangueil, Toulouse, France. souad.lachgar@pierre-fabre.com
FAU - Charveron, M
AU  - Charveron M
FAU - Sarraute, J
AU  - Sarraute J
FAU - Mourard, M
AU  - Mourard M
FAU - Gall, Y
AU  - Gall Y
FAU - Bonafe, J L
AU  - Bonafe JL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Investig Dermatol Symp Proc
JT  - The journal of investigative dermatology. Symposium proceedings
JID - 9609059
RN  - 0 (Androgens)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Estrogens)
RN  - 0 (Lymphokines)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
SB  - IM
MH  - Androgens/pharmacology
MH  - Animals
MH  - Cells, Cultured
MH  - Endothelial Growth Factors/*physiology
MH  - Estrogens/pharmacology
MH  - Gene Expression Regulation/physiology
MH  - Hair Follicle/blood supply/drug effects/*physiology
MH  - Humans
MH  - Lymphokines/*physiology
MH  - Mice
MH  - Neovascularization, Physiologic/*drug effects
MH  - Receptors, Androgen/*physiology
MH  - Receptors, Estrogen/*physiology
MH  - Signal Transduction/*drug effects
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
EDAT- 2000/02/16 09:00
MHDA- 2000/03/18 09:00
CRDT- 2000/02/16 09:00
PHST- 2000/02/16 09:00 [pubmed]
PHST- 2000/03/18 09:00 [medline]
PHST- 2000/02/16 09:00 [entrez]
AID - S1087-0024(15)30285-9 [pii]
AID - 10.1038/sj.jidsp.5640232 [doi]
PST - ppublish
SO  - J Investig Dermatol Symp Proc. 1999 Dec;4(3):290-5. doi: 
      10.1038/sj.jidsp.5640232.